Linked Life Data

16837648

Source:http://linkedlifedata.com/resource/pubmed/id/16837648

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-1-12
pubmed:abstractText
Interstitial cystitis (IC) is an idiopathic condition characterized by bladder hyperalgesia. Studies have shown cytokine and purinergic signaling abnormalities in cultured bladder urothelial cells (BUC) from IC patients. We performed single-cell electrophysiological studies in both normal and IC BUC. A strongly inward rectifying potassium current with conductance of the Kir2.1 channel was identified in normal BUC. This current was significantly reduced in IC BUC. Kir2.1 protein and mRNA were detected in both IC and normal BUC. Epidermal growth factor (EGF) caused a dose-dependent decrease in the inward potassium current in normal BUC. EGF is secreted in higher amounts by IC BUC and is known to decrease Kir2.1 conductance by phosphorylation of Kir2.1. Genistein, a nonspecific phosphorylation inhibitor, increased the inward potassium current in IC BUC and blocked the effect of EGF on normal BUC. Treatment of IC BUC with heparin-binding epidermal growth factor-like growth factor (HB-EGF), previously shown to be secreted in lower amounts by IC BUC, significantly increased inward potassium current. These data show that the inward potassium current in BUC can be modulated by EGF and HB-EGF. Changes in BUC membrane potassium conductance caused by altered levels of EGF and HB-EGF may therefore play a role in the pathophysiology of IC.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0363-6143
pubmed:author
pubmed:issnType
Print
pubmed:volume
292
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C106-14
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16837648-Cells, Cultured, pubmed-meshheading:16837648-Cystitis, Interstitial, pubmed-meshheading:16837648-Dose-Response Relationship, Drug, pubmed-meshheading:16837648-Electric Conductivity, pubmed-meshheading:16837648-Epidermal Growth Factor, pubmed-meshheading:16837648-Genistein, pubmed-meshheading:16837648-Humans, pubmed-meshheading:16837648-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:16837648-Large-Conductance Calcium-Activated Potassium Channels, pubmed-meshheading:16837648-Potassium Channels, pubmed-meshheading:16837648-Potassium Channels, Inwardly Rectifying, pubmed-meshheading:16837648-Protein Kinase Inhibitors, pubmed-meshheading:16837648-Protein-Tyrosine Kinases, pubmed-meshheading:16837648-Urinary Bladder, pubmed-meshheading:16837648-Urothelium
pubmed:year
2007
pubmed:articleTitle
EGF and HB-EGF modulate inward potassium current in human bladder urothelial cells from normal and interstitial cystitis patients.
pubmed:affiliation
Division of Urology, University of Maryland, 22 S. Greene Street, S8D18, Baltimore, MD 21201, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural