| pubmed-article:16835853 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16835853 | lifeskim:mentions | umls-concept:C0596901 | lld:lifeskim |
| pubmed-article:16835853 | lifeskim:mentions | umls-concept:C2347026 | lld:lifeskim |
| pubmed-article:16835853 | lifeskim:mentions | umls-concept:C0370003 | lld:lifeskim |
| pubmed-article:16835853 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:16835853 | lifeskim:mentions | umls-concept:C0751973 | lld:lifeskim |
| pubmed-article:16835853 | lifeskim:mentions | umls-concept:C0443199 | lld:lifeskim |
| pubmed-article:16835853 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:16835853 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:16835853 | pubmed:dateCreated | 2006-8-28 | lld:pubmed |
| pubmed-article:16835853 | pubmed:abstractText | Proteome studies with small sample amounts are difficult to perform, especially when membrane proteins are the focus of interest. In our study a new method for the analysis of scarce membrane protein samples combining large gel 2-D-CTAB/SDS-PAGE with fluorescence dye saturation labelling (satDIGE) was developed, allowing a highly sensitive differential analysis of different cell states. After Triton X-114 phase partitioning, enriched membrane protein samples of T cells were labelled at cysteine residues using fluorescence dyes and separated by large gel 2D-CTAB/SDS-PAGE. For a differential analysis 3 mug protein was found to be sufficient to detect proteins in a widespread well-separated diagonal spot pattern. | lld:pubmed |
| pubmed-article:16835853 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16835853 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16835853 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16835853 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16835853 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16835853 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16835853 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16835853 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16835853 | pubmed:issn | 1615-9853 | lld:pubmed |
| pubmed-article:16835853 | pubmed:author | pubmed-author:SchmittEdgarE | lld:pubmed |
| pubmed-article:16835853 | pubmed:author | pubmed-author:MarcusKatrinK | lld:pubmed |
| pubmed-article:16835853 | pubmed:author | pubmed-author:MeyerHelmut... | lld:pubmed |
| pubmed-article:16835853 | pubmed:author | pubmed-author:LutterPetraP | lld:pubmed |
| pubmed-article:16835853 | pubmed:author | pubmed-author:MüllnerStefan... | lld:pubmed |
| pubmed-article:16835853 | pubmed:author | pubmed-author:JoppichCornel... | lld:pubmed |
| pubmed-article:16835853 | pubmed:author | pubmed-author:BeckerGabriel... | lld:pubmed |
| pubmed-article:16835853 | pubmed:author | pubmed-author:SitekBarbaraB | lld:pubmed |
| pubmed-article:16835853 | pubmed:author | pubmed-author:SchulenborgTh... | lld:pubmed |
| pubmed-article:16835853 | pubmed:author | pubmed-author:HellingStefan... | lld:pubmed |
| pubmed-article:16835853 | pubmed:author | pubmed-author:Felske-Müller... | lld:pubmed |
| pubmed-article:16835853 | pubmed:author | pubmed-author:WiebringhausT... | lld:pubmed |
| pubmed-article:16835853 | pubmed:author | pubmed-author:LinsenmannGud... | lld:pubmed |
| pubmed-article:16835853 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16835853 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:16835853 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16835853 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16835853 | pubmed:pagination | 4506-13 | lld:pubmed |
| pubmed-article:16835853 | pubmed:meshHeading | pubmed-meshheading:16835853... | lld:pubmed |
| pubmed-article:16835853 | pubmed:meshHeading | pubmed-meshheading:16835853... | lld:pubmed |
| pubmed-article:16835853 | pubmed:meshHeading | pubmed-meshheading:16835853... | lld:pubmed |
| pubmed-article:16835853 | pubmed:meshHeading | pubmed-meshheading:16835853... | lld:pubmed |
| pubmed-article:16835853 | pubmed:meshHeading | pubmed-meshheading:16835853... | lld:pubmed |
| pubmed-article:16835853 | pubmed:meshHeading | pubmed-meshheading:16835853... | lld:pubmed |
| pubmed-article:16835853 | pubmed:meshHeading | pubmed-meshheading:16835853... | lld:pubmed |
| pubmed-article:16835853 | pubmed:meshHeading | pubmed-meshheading:16835853... | lld:pubmed |
| pubmed-article:16835853 | pubmed:meshHeading | pubmed-meshheading:16835853... | lld:pubmed |
| pubmed-article:16835853 | pubmed:meshHeading | pubmed-meshheading:16835853... | lld:pubmed |
| pubmed-article:16835853 | pubmed:meshHeading | pubmed-meshheading:16835853... | lld:pubmed |
| pubmed-article:16835853 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16835853 | pubmed:articleTitle | 2-D differential membrane proteome analysis of scarce protein samples. | lld:pubmed |
| pubmed-article:16835853 | pubmed:affiliation | Medizinisches Proteom-Center, Ruhr-Universität Bochum, Bochum, Germany. | lld:pubmed |
| pubmed-article:16835853 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16835853 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16835853 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16835853 | lld:pubmed |
