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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
2006-8-28
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pubmed:abstractText |
Proteome studies with small sample amounts are difficult to perform, especially when membrane proteins are the focus of interest. In our study a new method for the analysis of scarce membrane protein samples combining large gel 2-D-CTAB/SDS-PAGE with fluorescence dye saturation labelling (satDIGE) was developed, allowing a highly sensitive differential analysis of different cell states. After Triton X-114 phase partitioning, enriched membrane protein samples of T cells were labelled at cysteine residues using fluorescence dyes and separated by large gel 2D-CTAB/SDS-PAGE. For a differential analysis 3 mug protein was found to be sufficient to detect proteins in a widespread well-separated diagonal spot pattern.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1615-9853
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pubmed:author |
pubmed-author:BeckerGabrieleG,
pubmed-author:Felske-MüllerStephanieS,
pubmed-author:HellingStefanS,
pubmed-author:JoppichCorneliaC,
pubmed-author:LinsenmannGudrunG,
pubmed-author:LutterPetraP,
pubmed-author:MüllnerStefanS,
pubmed-author:MarcusKatrinK,
pubmed-author:MeyerHelmut EHE,
pubmed-author:SchmittEdgarE,
pubmed-author:SchulenborgThomasT,
pubmed-author:SitekBarbaraB,
pubmed-author:WiebringhausThomasT
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4506-13
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pubmed:meshHeading |
pubmed-meshheading:16835853-Amino Acid Sequence,
pubmed-meshheading:16835853-Animals,
pubmed-meshheading:16835853-Cells, Cultured,
pubmed-meshheading:16835853-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:16835853-Fluorescent Dyes,
pubmed-meshheading:16835853-Humans,
pubmed-meshheading:16835853-Membrane Proteins,
pubmed-meshheading:16835853-Mice,
pubmed-meshheading:16835853-Molecular Sequence Data,
pubmed-meshheading:16835853-Proteome,
pubmed-meshheading:16835853-Spectrometry, Mass, Electrospray Ionization
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pubmed:year |
2006
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pubmed:articleTitle |
2-D differential membrane proteome analysis of scarce protein samples.
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pubmed:affiliation |
Medizinisches Proteom-Center, Ruhr-Universität Bochum, Bochum, Germany.
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pubmed:publicationType |
Journal Article
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