16765841

Source:http://linkedlifedata.com/resource/pubmed/id/16765841

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0023238, umls-concept:C0206691, umls-concept:C1510411
pubmed:issue	3
pubmed:dateCreated	2006-6-12
pubmed:abstractText	A simple and reproducible method has been developed to transform Legionella pneumophila by electroporation. Effects of different conditions, including electric field strength, pulse length, DNA quality and cell density, were evaluated. Using our method, an efficiency of up to 6 x 10(7) transformants/microg DNA was obtained. This optimized transformation procedure should efficiently facilitate gene manipulations in L. pneumophila, such as plasmid transfer, transposon mutagenesis, library transformation for complementation cloning, etc.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/9437794
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Edetic Acid
pubmed:status	MEDLINE
pubmed:issn	0944-5013
pubmed:author	pubmed-author:ChenDing-QiangDQ, pubmed-author:HuangShao-SongSS, pubmed-author:LuYong-JunYJ
pubmed:issnType	Print
pubmed:volume	161
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	246-51
pubmed:dateRevised	2006-11-15
pubmed:meshHeading	pubmed-meshheading:16765841-Edetic Acid, pubmed-meshheading:16765841-Electroporation, pubmed-meshheading:16765841-Legionella pneumophila, pubmed-meshheading:16765841-Plasmids, pubmed-meshheading:16765841-Transformation, Bacterial
pubmed:year	2006
pubmed:articleTitle	Efficient transformation of Legionella pneumophila by high-voltage electroporation.
pubmed:affiliation	Department of Biochemistry and State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen Zhongshan University, Guangzhou, China.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't