Linked Life Data

16754516

Source:http://linkedlifedata.com/resource/pubmed/id/16754516

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2006-6-6
pubmed:abstractText
A biological and embryological bone induction from epithelial-mesenchymal cell interactions has been noticed in some developing tissues. However, the mechanism for bone formation induced by the epithelial-mesenchymal cell interactions is not clear. The aim of our study was to reveal the role of laminin, vascular endothelial growth factor (VEGF), and bone matrix proteins in mesenchymal cell differentiation during uroepithelial bone induction using a well-established canine model. In this model, a myoperitoneal muscle flap from the abdominal rectus sheath was transplanted into the bladder wall. After 6 weeks, the bladder samples were removed and assessed by histology and immunohistochemistry. This study demonstrated that bone formation occurred in two different directions with two distinct mechanisms. We noted that bone-forming cells in two types of bone formation derived from mesenchymal stem cell differentiation induced either from uroepithelium or bone autoinduction. Laminin was only expressed in peripheral regions of uroepithelium bone formation. Type II collagen was expressed both intracellularly and extracellularly around hypertrophic chondrocytes, whereas VEGF was mostly expressed in proliferating chondrocytes. This study indicates that components in basement membrane like laminin play a role in transitional epithelium-induced differentiation of mesenchymal cells to chondrocytes in muscle tissue. The sequential expression of bone matrix proteins by differentiated osteogenetic cells indicates a subsequent sequence of bone autoinduction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0300-8207
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
102-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16754516-Animals, pubmed-meshheading:16754516-Bone Morphogenetic Protein 2, pubmed-meshheading:16754516-Bone Morphogenetic Protein 4, pubmed-meshheading:16754516-Bone Morphogenetic Proteins, pubmed-meshheading:16754516-Cell Differentiation, pubmed-meshheading:16754516-Cell Proliferation, pubmed-meshheading:16754516-Chondrocytes, pubmed-meshheading:16754516-Collagen Type II, pubmed-meshheading:16754516-Dogs, pubmed-meshheading:16754516-Female, pubmed-meshheading:16754516-Laminin, pubmed-meshheading:16754516-Models, Animal, pubmed-meshheading:16754516-Osteogenesis, pubmed-meshheading:16754516-Transforming Growth Factor beta, pubmed-meshheading:16754516-Transplantation, Homologous, pubmed-meshheading:16754516-Urothelium, pubmed-meshheading:16754516-Vascular Endothelial Growth Factor A
pubmed:year
2006
pubmed:articleTitle
Laminin, VEGF, and bone matrix protein expression in uroepithelial bone induction--a canine model.
pubmed:affiliation
AOSpine Reference Centre, School of Engineering Systems, Queensland University of Technology, Brisbane, Australia. yin.xiao@qut.edu.au
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't