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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2006-9-11
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pubmed:abstractText |
Soluble glucocorticoid-induced tumor necrosis factor receptor (sGITR) is a potent stimulator of osteoclastogenesis. The mechanism by which it induces osteoclastogenesis was studied by culturing bone-marrow-derived macrophages (BMM) with conditioned medium from mouse bone marrow stromal cells. GITR and GITR ligand (GITRL) were expressed on the surface of bone marrow stromal cells, and sGITR-induced osteoclastogenesis was inhibited by anti-GITRL Ab, indicating that stimulatory effect of osteoclastogenesis by sGITR involved signaling via GITRL. Bone marrow stromal cells up-regulated cyclooxygenase-2 (COX-2) and produced prostaglandin E(2) (PGE(2)) early in their response to sGITR, and the stimulation of osteoclastogenesis was markedly inhibited by NS398, a COX-2 inhibitor. Later, sGITR markedly reduced the steady-state level of osteoprotegerin (OPG) mRNA and increased receptor activator of nuclear factor-kappaB ligand (RANKL) mRNA. NS398 blocked the sGITR-induced reduction of OPG mRNA but did not significantly affect the sGITR-induced rise in RANKL mRNA. This suggests that down-regulation of OPG by PGE(2) is involved in sGITR-induced osteoclast (OC) formation in the presence of conditioned medium from mouse bone marrow stromal cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2 Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Glucocorticoid-Induced...,
http://linkedlifedata.com/resource/pubmed/chemical/Osteoprotegerin,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandins E,
http://linkedlifedata.com/resource/pubmed/chemical/RANK Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfrsf18 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfsf18 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
8756-3282
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
716-23
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16750437-Animals,
pubmed-meshheading:16750437-Antibodies,
pubmed-meshheading:16750437-Bone Marrow Cells,
pubmed-meshheading:16750437-Cell Line,
pubmed-meshheading:16750437-Cells, Cultured,
pubmed-meshheading:16750437-Cyclooxygenase 2,
pubmed-meshheading:16750437-Cyclooxygenase 2 Inhibitors,
pubmed-meshheading:16750437-Dose-Response Relationship, Drug,
pubmed-meshheading:16750437-Down-Regulation,
pubmed-meshheading:16750437-Flow Cytometry,
pubmed-meshheading:16750437-Gene Expression,
pubmed-meshheading:16750437-Glucocorticoid-Induced TNFR-Related Protein,
pubmed-meshheading:16750437-Mice,
pubmed-meshheading:16750437-Mice, Inbred C57BL,
pubmed-meshheading:16750437-Osteoclasts,
pubmed-meshheading:16750437-Osteoprotegerin,
pubmed-meshheading:16750437-Prostaglandins E,
pubmed-meshheading:16750437-RANK Ligand,
pubmed-meshheading:16750437-Receptors, Nerve Growth Factor,
pubmed-meshheading:16750437-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:16750437-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16750437-Stromal Cells,
pubmed-meshheading:16750437-Tumor Necrosis Factors
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pubmed:year |
2006
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pubmed:articleTitle |
Soluble glucocorticoid-induced tumor necrosis factor receptor stimulates osteoclastogenesis by down-regulation of osteoprotegerin in bone marrow stromal cells.
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pubmed:affiliation |
Department of Biological Sciences and the Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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