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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2006-11-3
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pubmed:abstractText |
Lysosomal cysteine protease cathepsin B has been reported to play an important role in apoptosis of many different cancer cells, but the regulation of cathepsin B in apoptosis is poorly understood. Human homologue of SETA binding protein 1 (hSB1) was identified to interact with cathepsin B by yeast-two hybrid method, and the interaction was confirmed in vitro GST pull-down assay and in vivo coimmunoprecipitation experiment. hSB1 was co-localized with cathepsin B in cellular lysosomes. Our previous study has shown that TNF can induce ovarian cancer cells OV-90 apoptosis and the apoptosis process is cathepsin B-depended. Here we provide evidence that overexpression of cathepsin B-interacting protein hSB1 could suppress TNF-triggered apoptosis in OV-90 cells, but has no effect on cellular cathepsin B activity. hSB1 may function as a regulator of cathepsin B-mediated apoptosis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0300-8177
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
292
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
189-95
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pubmed:meshHeading |
pubmed-meshheading:16733801-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:16733801-Apoptosis,
pubmed-meshheading:16733801-Cathepsin B,
pubmed-meshheading:16733801-Female,
pubmed-meshheading:16733801-Gene Expression,
pubmed-meshheading:16733801-Gene Expression Profiling,
pubmed-meshheading:16733801-Humans,
pubmed-meshheading:16733801-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:16733801-Lysosomes,
pubmed-meshheading:16733801-Ovarian Neoplasms,
pubmed-meshheading:16733801-Protein Binding,
pubmed-meshheading:16733801-Protein Transport,
pubmed-meshheading:16733801-Reproducibility of Results,
pubmed-meshheading:16733801-Sequence Homology,
pubmed-meshheading:16733801-Tumor Necrosis Factor-alpha,
pubmed-meshheading:16733801-Two-Hybrid System Techniques
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pubmed:year |
2006
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pubmed:articleTitle |
Human homologue of SETA binding protein 1 interacts with cathepsin B and participates in TNF-Induced apoptosis in ovarian cancer cells.
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pubmed:affiliation |
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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