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rdf:type |
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lifeskim:mentions |
umls-concept:C0007603,
umls-concept:C0008550,
umls-concept:C0023516,
umls-concept:C0026336,
umls-concept:C0220908,
umls-concept:C0449432,
umls-concept:C1179435,
umls-concept:C1411976,
umls-concept:C1500734,
umls-concept:C1524073,
umls-concept:C1548799,
umls-concept:C1705248
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pubmed:issue |
2
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pubmed:dateCreated |
2006-5-17
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pubmed:abstractText |
A model of white blood cell membrane chromatography (WB-CMC) was established to screen active component from Atractylodes macrocephala Koidz. The component can antagonize Toll-like receptor 4 (TLR4) and inhibit inflammatory reaction. In the model of WB-CMC, cell membrane stationary phase (CMSP) was prepared by immobilizing the rabbit white blood cell membrane (WBCM) onto the surface of silica carrier and taxinol was used as a model molecule. The active component which can act on WBCM and its receptor (such as TLR4) as an effective target in A. macrocephala was determined by using a replacement experiment. The anti-inflammatory effects of the active component were tested by using pharmacological methods in vivo. The results indicated that the retention characteristics of atractylenolide I as active component was similar to that of taxinol in the model of WB-CMC. And so, atractylenolide I acted on the WBCM and TLR4 and its anti-inflammatory activity was related with antagonizing TLR4. Therefore, the interaction between the active component and WBCM and its receptor can be simulated by the model of WB-CMC in vitro. This model can be used to screen active components and to study effective characteristics for acting on definite targets.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1006-9305
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
182-9
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pubmed:meshHeading |
pubmed-meshheading:16704122-Acetic Acid,
pubmed-meshheading:16704122-Animals,
pubmed-meshheading:16704122-Anti-Inflammatory Agents,
pubmed-meshheading:16704122-Atractylodes,
pubmed-meshheading:16704122-Cell Membrane,
pubmed-meshheading:16704122-Chromatography,
pubmed-meshheading:16704122-Ear Diseases,
pubmed-meshheading:16704122-Edema,
pubmed-meshheading:16704122-Female,
pubmed-meshheading:16704122-Lactones,
pubmed-meshheading:16704122-Leukocytes,
pubmed-meshheading:16704122-Male,
pubmed-meshheading:16704122-Mice,
pubmed-meshheading:16704122-Microscopy, Electron, Scanning,
pubmed-meshheading:16704122-Peritonitis,
pubmed-meshheading:16704122-Plant Extracts,
pubmed-meshheading:16704122-Rabbits,
pubmed-meshheading:16704122-Reproducibility of Results,
pubmed-meshheading:16704122-Sesquiterpenes,
pubmed-meshheading:16704122-Toll-Like Receptor 4,
pubmed-meshheading:16704122-Xylenes
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pubmed:year |
2006
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pubmed:articleTitle |
Establishment of the model of white blood cell membrane chromatography and screening of antagonizing TLR4 receptor component from Atractylodes macrocephala Koidz.
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pubmed:affiliation |
School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
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pubmed:publicationType |
Journal Article
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