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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 6
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pubmed:dateCreated |
2006-5-12
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pubmed:abstractText |
Genetic immunization using viral vectors provides an effective means to elicit antigen-specific cellular immune responses. Several viral vectors have proven efficacious in inducing immune responses after direct injection in vivo. Among them, recombinant, self-inactivating lentiviral vectors are very attractive delivery systems, as they are able to efficiently transduce into and express foreign genes in a wide variety of mammalian cells. A self-inactivating lentiviral vector was evaluated for the delivery of human immunodeficiency virus 1 (HIV-1) envelope sequences in mice in order to elicit specific immune responses. With this aim, BALB/c mice were immunized with a single injection of self-inactivating lentiviral vectors carrying either the full-length HIV-1(HXB2) Rev/Env (TY2-IIIBEnv) or the codon-optimized HIV-1(JR-FL) gp120 (TY2-JREnv) coding sequence. Both vectors were able to elicit specific cellular responses efficiently, as measured by gamma interferon ELISPOT and chromium-release assays, upon in vitro stimulation of splenocytes from BALB/c immunized mice. However, only the TY2-JREnv-immunized mice were able to elicit specific humoral responses, measured as anti-gp120 antibody production. These data provide the first evidence that a single, direct, in vivo administration of a lentiviral vector encoding a viral gene might represent a useful strategy for vaccine development.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AIDS Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Codon,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, env,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, rev,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp120,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/rev Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1625-34
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16690927-AIDS Vaccines,
pubmed-meshheading:16690927-Animals,
pubmed-meshheading:16690927-Codon,
pubmed-meshheading:16690927-Female,
pubmed-meshheading:16690927-Gene Products, env,
pubmed-meshheading:16690927-Gene Products, rev,
pubmed-meshheading:16690927-Genes, env,
pubmed-meshheading:16690927-Genes, rev,
pubmed-meshheading:16690927-Genetic Vectors,
pubmed-meshheading:16690927-HIV Antibodies,
pubmed-meshheading:16690927-HIV Envelope Protein gp120,
pubmed-meshheading:16690927-HIV Infections,
pubmed-meshheading:16690927-HIV-1,
pubmed-meshheading:16690927-Humans,
pubmed-meshheading:16690927-Immunization,
pubmed-meshheading:16690927-Injections, Intramuscular,
pubmed-meshheading:16690927-Interferon-gamma,
pubmed-meshheading:16690927-Lentivirus,
pubmed-meshheading:16690927-Mice,
pubmed-meshheading:16690927-Mice, Inbred BALB C,
pubmed-meshheading:16690927-T-Lymphocytes,
pubmed-meshheading:16690927-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:16690927-rev Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2006
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pubmed:articleTitle |
A single administration of lentiviral vectors expressing either full-length human immunodeficiency virus 1 (HIV-1)(HXB2) Rev/Env or codon-optimized HIV-1(JR-FL) gp120 generates durable immune responses in mice.
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pubmed:affiliation |
National AIDS Center, Department of Drugs and Evaluation, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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