|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
10
|
|
pubmed:dateCreated |
1992-10-16
|
|
pubmed:abstractText |
Epstein-Barr virus (EBV)-specific DNA sequences were detected by polymerase chain reaction analysis in 15 of 47 (32%) DNA extracts prepared from CD30-positive (Ki-1 antigen-positive) anaplastic large cell (ALC) lymphomas. EBV-encoded RNA (EBER) transcripts could be detected by in situ hybridization in the tumor cells of 9 of 11 EBV DNA-positive cases. Twenty-eight cases were examined by immunohistology on cryostat sections for the presence of the EBV-encoded latent membrane protein (LMP), the nuclear antigen 2 (EBNA2), the BZLF1 transactivator protein, and the late viral glycoprotein gp350/250. A distinct LMP-specific membrane and cytoplasmic staining was detected exclusively in lymphoma cells of five cases (18%); two of these cases additionally expressed EBNA2. BZLF1 protein and gp350/250 immunoreactivity was absent in all instances. All LMP-positive cases contained EBV DNA and EBER sequences. The pattern of EBV latent protein expression in ALC lymphomas showed heterogeneity with respect to EBNA2 expression: LMP-positive/EBNA2-negative cases displayed a pattern previously described for undifferentiated nasopharyngeal carcinomas and Hodgkin's disease, whereas LMP-positive and EBNA2-positive cases showed parallels to lymphoblastoid cell lines. Because the LMP gene has transforming potential, our findings support the concept of a pathoetiologic role for EBV in a proportion of CD30-positive ALC lymphomas.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD30,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Epstein-Barr Virus Nuclear Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0006-4971
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
78
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2666-73
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:1668608-Adult,
pubmed-meshheading:1668608-Aged,
pubmed-meshheading:1668608-Antigens, CD,
pubmed-meshheading:1668608-Antigens, CD30,
pubmed-meshheading:1668608-Antigens, Neoplasm,
pubmed-meshheading:1668608-Antigens, Viral,
pubmed-meshheading:1668608-Base Sequence,
pubmed-meshheading:1668608-DNA, Viral,
pubmed-meshheading:1668608-DNA-Binding Proteins,
pubmed-meshheading:1668608-Epstein-Barr Virus Nuclear Antigens,
pubmed-meshheading:1668608-Female,
pubmed-meshheading:1668608-Genes, Viral,
pubmed-meshheading:1668608-Genome, Viral,
pubmed-meshheading:1668608-Herpesvirus 4, Human,
pubmed-meshheading:1668608-Humans,
pubmed-meshheading:1668608-Immunophenotyping,
pubmed-meshheading:1668608-Lymph Nodes,
pubmed-meshheading:1668608-Lymphoma, Large B-Cell, Diffuse,
pubmed-meshheading:1668608-Male,
pubmed-meshheading:1668608-Middle Aged,
pubmed-meshheading:1668608-Molecular Sequence Data,
pubmed-meshheading:1668608-Oligodeoxyribonucleotides,
pubmed-meshheading:1668608-Polymerase Chain Reaction,
pubmed-meshheading:1668608-Restriction Mapping
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Epstein-Barr virus DNA and latent gene products in Ki-1 (CD30)-positive anaplastic large cell lymphomas.
|
|
pubmed:affiliation |
Institute of Pathology, Klinikum Steglitz, Free University of Berlin, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
