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rdf:type |
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lifeskim:mentions |
umls-concept:C0002245,
umls-concept:C0029045,
umls-concept:C0035668,
umls-concept:C0040711,
umls-concept:C0043343,
umls-concept:C0220781,
umls-concept:C0934502,
umls-concept:C0982459,
umls-concept:C1095911,
umls-concept:C1123020,
umls-concept:C1533691,
umls-concept:C1709694,
umls-concept:C1883254
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pubmed:issue |
1
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pubmed:dateCreated |
2010-6-29
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pubmed:abstractText |
Wheat (Triticum aestivum) RNA was used to program synthesis of the alpha-amylase protein by Xenopus laevis oocytes. A 41,500-dalton protein was made which was identified as alpha-amylase by immunoprecipitation with rabbit anti-alpha-amylase antiserum raised against the purified wheat protein and by its co-migration with authentic alpha-amylase on sodium dodecyl sulfate polyacrylamide gels. Synthesis of alpha-amylase was dependent upon injection of RNA extracted from gibberellic acid-induced aleurone layers from wheat. The amount of alpha-amylase produced was proportional to the amount of RNA injected and reached a plateau within 4 hours after injection. When the same RNA was translated in a wheat germ cell-free translation system, a 43,000-dalton protein was produced. Addition of dog pancreas microsomal membranes to the wheat germ translation system resulted in processing of the alpha-amylase protein to a form which co-migrated with authentic alpha-amylase purified from malted wheat and with the protein synthesized in oocytes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-1010977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-1102604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-1236796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-16592743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-16660677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-16661291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-16661647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-198138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-198778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-288049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-293711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-325565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-332519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-4345506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-4525168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-4528576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-477987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-500726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-5237885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-5339108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-537089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-568137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-6156008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-6162638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-649601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-668692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-6987533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-7193578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-7251653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-7408891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-7439186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-811671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-823012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662149-836272
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pubmed:language |
eng
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pubmed:journal |
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pubmed:status |
PubMed-not-MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0032-0889
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
150-4
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pubmed:dateRevised |
2010-9-14
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pubmed:year |
1982
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pubmed:articleTitle |
In Vitro Synthesis and Processing of Wheat alpha-Amylase : TRANSLATION OF GIBBERELLIC ACID-INDUCED WHEAT ALEURONE LAYER RNA BY WHEAT GERM AND XENOPUS LAEVIS OOCYTE SYSTEMS.
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pubmed:affiliation |
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin 53706.
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pubmed:publicationType |
Journal Article
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