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pubmed-article:1663889pubmed:abstractTextThe class 3 protein (PorB) is an important component of the meningococcal outer membrane. The structural gene (porB) encoding the class 3 protein has been cloned using primers suitable for the amplification of the corresponding chromosomal fragment by the polymerase chain reaction (PCR). The complete nucleotide sequence was determined and predicts a mature protein of 310 amino acids, preceded by a signal peptide of 19 residues. The predicted protein sequence of the class 3 protein exhibits essential structural homology to the gonococcal porin PIA. The class 3 protein encoding gene was expressed in Escherichia coli under the control of an inducible promoter.lld:pubmed
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pubmed-article:1663889pubmed:articleTitleThe class 3 outer membrane protein (PorB) of Neisseria meningitidis: gene sequence and homology to the gonococcal porin PIA.lld:pubmed
pubmed-article:1663889pubmed:affiliationDepartment of Genetics, Boehringer Mannheim GmbH, Penzberg, F.R.G.lld:pubmed
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