rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
1992-3-9
|
pubmed:databankReference |
|
pubmed:abstractText |
The class 3 protein (PorB) is an important component of the meningococcal outer membrane. The structural gene (porB) encoding the class 3 protein has been cloned using primers suitable for the amplification of the corresponding chromosomal fragment by the polymerase chain reaction (PCR). The complete nucleotide sequence was determined and predicts a mature protein of 310 amino acids, preceded by a signal peptide of 19 residues. The predicted protein sequence of the class 3 protein exhibits essential structural homology to the gonococcal porin PIA. The class 3 protein encoding gene was expressed in Escherichia coli under the control of an inducible promoter.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
|
pubmed:issn |
0378-1097
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
67
|
pubmed:geneSymbol |
porB
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
179-85
|
pubmed:dateRevised |
2011-4-7
|
pubmed:meshHeading |
pubmed-meshheading:1663889-Amino Acid Sequence,
pubmed-meshheading:1663889-Bacterial Outer Membrane Proteins,
pubmed-meshheading:1663889-Base Sequence,
pubmed-meshheading:1663889-Gene Expression,
pubmed-meshheading:1663889-Genes, Bacterial,
pubmed-meshheading:1663889-Molecular Sequence Data,
pubmed-meshheading:1663889-Neisseria gonorrhoeae,
pubmed-meshheading:1663889-Neisseria meningitidis,
pubmed-meshheading:1663889-Oligodeoxyribonucleotides,
pubmed-meshheading:1663889-Polymerase Chain Reaction,
pubmed-meshheading:1663889-Porins,
pubmed-meshheading:1663889-Sequence Homology, Nucleic Acid
|
pubmed:year |
1991
|
pubmed:articleTitle |
The class 3 outer membrane protein (PorB) of Neisseria meningitidis: gene sequence and homology to the gonococcal porin PIA.
|
pubmed:affiliation |
Department of Genetics, Boehringer Mannheim GmbH, Penzberg, F.R.G.
|
pubmed:publicationType |
Journal Article,
Comparative Study
|