Source:http://linkedlifedata.com/resource/pubmed/id/16630542
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0006675,
umls-concept:C0007634,
umls-concept:C0026724,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0086045,
umls-concept:C0109317,
umls-concept:C0178719,
umls-concept:C0752312,
umls-concept:C1150579,
umls-concept:C1333340,
umls-concept:C1335824,
umls-concept:C1366882,
umls-concept:C1370600,
umls-concept:C1514485,
umls-concept:C1704242,
umls-concept:C1705767,
umls-concept:C1705791,
umls-concept:C1879547
|
| pubmed:issue |
2
|
| pubmed:dateCreated |
2006-5-1
|
| pubmed:abstractText |
Sonic Hedgehog (Shh), a member of hedgehog peptides family, is expressed in gastric gland epithelium. To elucidate Shh function to gastric mucosal cells, we examined the effect of Shh on the proliferation of a rat normal gastric mucosal cell line, RGM-1. RGM-1 cells express essential components of Shh receptor system, patched-1, and smoothened. Shh enhanced DNA synthesis in RGM-1 cells and elevated intracellular calcium concentration ([Ca2+]i). In addition, Shh as well as calcium ionophore A32187 rapidly activated ERK. However, Shh failed to activate ERK under calcium-free culture condition. Pretreatment of cells with PD98059 attenuated the DNA synthesis promoted by Shh. Moreover, when cells were pretreated with cyclopamine, Shh could not elevate [Ca2+]i, activate ERK or promote DNA synthesis. On the other hand, although Shh induced Gli-1 nuclear accumulation in RGM-1 cells, Shh activated ERK even in cells pretreated with actinomycin D. These results indicate that Shh promotes the proliferation of RGM-1 cells through an intracellular calcium- and ERK-dependent but transcription-independent pathway via Patched/Smoothened receptor system.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
2
|
| pubmed:volume |
344
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
680-7
|
| pubmed:dateRevised |
2010-4-12
|
| pubmed:meshHeading |
pubmed-meshheading:16630542-Animals,
pubmed-meshheading:16630542-Calcium,
pubmed-meshheading:16630542-Cell Line,
pubmed-meshheading:16630542-Cell Proliferation,
pubmed-meshheading:16630542-Dose-Response Relationship, Drug,
pubmed-meshheading:16630542-Enzyme Activation,
pubmed-meshheading:16630542-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:16630542-Gastric Mucosa,
pubmed-meshheading:16630542-Hedgehog Proteins,
pubmed-meshheading:16630542-Intracellular Fluid,
pubmed-meshheading:16630542-MAP Kinase Signaling System,
pubmed-meshheading:16630542-Rats,
pubmed-meshheading:16630542-Trans-Activators
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Sonic hedgehog stimulates the proliferation of rat gastric mucosal cells through ERK activation by elevating intracellular calcium concentration.
|
| pubmed:affiliation |
Department of Gastroenterology, Jichi Medical School, Tochigi 329-0498, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|