Source:http://linkedlifedata.com/resource/pubmed/id/16606368
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2006-5-11
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| pubmed:abstractText |
N-acetylglucosaminyltransferase VB (GnT-VB, -IX) is a newly discovered glycosyltransferase expressed exclusively in high levels in neuronal tissue during early development. Its homolog, GnT-V, is expressed in many tissues and modulates cell-cell and cell-matrix adhesion. The ability of GnT-VB to regulate cell-matrix interactions was initially investigated using the rat pheochromocytoma PC12 neurite outgrowth model. PC12 cells stably transfected with GnT-VB consistently showed an enhanced rate of nerve growth factor (NGF)-induced neurite outgrowth on collagen and laminin substrates. Levels of TrkA receptor phosphorylation and downstream ERK activation induced by NGF were not influenced by GnT-VB expression. No significant difference was observed in the rate of neurite outgrowth when cells were cultured on non-coated culture dishes, indicating that integrin-ECM interaction is required for the stimulatory effects. Neurite outgrowth induced by manganese-dependent activation of beta1 integrin on collagen and laminin substrates, however, showed a significant increase in neurite length for the PC12/GnT-VB cells, compared with control cells, suggesting that the enhancement is most likely mediated by alteration of beta1 integrin-ECM interaction by GnT-VB. These results demonstrate that GnT-VB expression can modulate the rate of neurite outgrowth by affecting beta1 integrin-ECM interaction.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD29,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Laminin,
http://linkedlifedata.com/resource/pubmed/chemical/MGAT5B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Manganese,
http://linkedlifedata.com/resource/pubmed/chemical/N-Acetylglucosaminyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, trkA
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| pubmed:status |
MEDLINE
|
| pubmed:month |
May
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| pubmed:issn |
0022-3042
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
97
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
947-56
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:16606368-Animals,
pubmed-meshheading:16606368-Antigens, CD29,
pubmed-meshheading:16606368-Cell Differentiation,
pubmed-meshheading:16606368-Cell Line, Tumor,
pubmed-meshheading:16606368-Cell Movement,
pubmed-meshheading:16606368-Collagen,
pubmed-meshheading:16606368-Enzyme Activation,
pubmed-meshheading:16606368-Extracellular Matrix,
pubmed-meshheading:16606368-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:16606368-Glycosylation,
pubmed-meshheading:16606368-Humans,
pubmed-meshheading:16606368-Laminin,
pubmed-meshheading:16606368-Manganese,
pubmed-meshheading:16606368-N-Acetylglucosaminyltransferases,
pubmed-meshheading:16606368-Nerve Tissue Proteins,
pubmed-meshheading:16606368-Neurites,
pubmed-meshheading:16606368-PC12 Cells,
pubmed-meshheading:16606368-Rats,
pubmed-meshheading:16606368-Receptor, trkA,
pubmed-meshheading:16606368-Transfection,
pubmed-meshheading:16606368-Up-Regulation
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| pubmed:year |
2006
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| pubmed:articleTitle |
N-acetylglucosaminyltranferase VB expression enhances beta1 integrin- dependent PC12 neurite outgrowth on laminin and collagen.
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| pubmed:affiliation |
The Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, the University of Georgia, Athens, Georgia 30602-4712, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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