Source:http://linkedlifedata.com/resource/pubmed/id/16604526
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2006-4-17
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| pubmed:abstractText |
We constructed a cyclooxygenase-2 (Cox-2) conditional overexpression transgenic mouse (Cox-2 COE). The transgene contains a CAG promoter driving the Cox-2 and humanized Renilla luciferase (hRL) coding regions, linked by an internal ribosomal entry site. The promoter is followed by a loxP-flanked sequence containing enhanced green fluorescent protein (EGFP), a neomycin selection cassette, and a transcriptional/translational STOP sequence. In the presence of Cre recombinase the loxP-flanked sequence is excised. Cox-2/hRL expression can be monitored repeatedly and noninvasively in vivo by imaging hRL activity. To demonstrate conditional Cox-2 and hRL expression, a nonreplicating adenovirus carrying Cre recombinase (Ad.CMV.Cre) was injected intravenously; hepatic Cox-2 expression and hRL signal were elevated. Cox-2 COE embryonic fibroblasts express both Cox-2 and hRL following Ad.CMV.Cre infection. PGE(2) production is also increased following Ad.CMV.Cre infection of Cox-2 COE embryo fibroblasts. Cox-2 COE mice should be valuable for the study of Cox-2 overexpression in cardiovascular disease, acute and chronic inflammatory responses, neurodegenerative diseases, and cancer.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cre recombinase,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Integrases,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases, Renilla,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
1526-954X
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| pubmed:author | |
| pubmed:copyrightInfo |
Published 2006 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:volume |
44
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
177-82
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:16604526-Adenoviridae,
pubmed-meshheading:16604526-Animals,
pubmed-meshheading:16604526-Cyclooxygenase 2,
pubmed-meshheading:16604526-Fibroblasts,
pubmed-meshheading:16604526-Gene Deletion,
pubmed-meshheading:16604526-Gene Expression Regulation,
pubmed-meshheading:16604526-Genetic Techniques,
pubmed-meshheading:16604526-Genetic Vectors,
pubmed-meshheading:16604526-Green Fluorescent Proteins,
pubmed-meshheading:16604526-Injections, Intravenous,
pubmed-meshheading:16604526-Integrases,
pubmed-meshheading:16604526-Liver,
pubmed-meshheading:16604526-Luciferases, Renilla,
pubmed-meshheading:16604526-Mice,
pubmed-meshheading:16604526-Mice, Transgenic,
pubmed-meshheading:16604526-Models, Genetic,
pubmed-meshheading:16604526-Promoter Regions, Genetic,
pubmed-meshheading:16604526-Transgenes,
pubmed-meshheading:16604526-Viral Proteins
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| pubmed:year |
2006
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| pubmed:articleTitle |
Transgenic mouse for conditional, tissue-specific Cox-2 overexpression.
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| pubmed:affiliation |
Department of Molecular and Medical Pharmacology, Molecular Biology Institute, Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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