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rdf:type |
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lifeskim:mentions |
umls-concept:C0001962,
umls-concept:C0015264,
umls-concept:C0079904,
umls-concept:C0441472,
umls-concept:C0752312,
umls-concept:C1136246,
umls-concept:C1370600,
umls-concept:C1555707,
umls-concept:C1704259,
umls-concept:C1705851,
umls-concept:C1705987,
umls-concept:C2752151,
umls-concept:C2828366,
umls-concept:C2930155
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|
pubmed:issue |
5
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pubmed:dateCreated |
2006-4-5
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|
pubmed:abstractText |
Artemisia vestita Wall., a traditional Tibetan medicine, has wide clinical application for inflammatory diseases. However, its molecular mechanism of anti-inflammatory effect is poorly understood. In the present study, we investigated the anti-inflammatory activity and underlying mechanism of the ethanol extract from Artemisia vestita (AV-ext) on lipopolysaccharide (LPS)-induced sepsis. Pretreatment with AV-ext significantly decreased the levels of tumor necrosis factor-alpha (TNF-alpha) in serum and liver and lung tissues, and improved the survival of mice with experimental sepsis. AV-ext also remarkably reduced the expression levels of TNF-alpha, interleukin-1beta and cyclooxygenase-2 in LPS-stimulated RAW 264.7 macrophages and dose dependently suppressed the activation of mitogen-activated protein kinases (MAPKs), such as p38, extracellular signal-regulated kinase (ERK1/2) and c-Jun NH2-terminal kinase (JNK). Furthermore, pretreatment with AV-ext dose dependently inhibited the activation of nuclear factor-kappaB (NF-kappaB), as well as the degradation and phosphorylation of inhibitory kappaB (IkappaB) in LPS-activated RAW 264.7 macrophages. These results collectively reveal that AV-ext inhibits TNF-alpha release from macrophages by suppressing MAPK and NF-kappaB signaling pathways and suggest that AV-ext may be beneficial for the treatment of endotoxin shock or sepsis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1107-3756
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
957-62
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:16596287-Animals,
pubmed-meshheading:16596287-Artemisia,
pubmed-meshheading:16596287-Cell Line,
pubmed-meshheading:16596287-Cyclooxygenase 2,
pubmed-meshheading:16596287-Down-Regulation,
pubmed-meshheading:16596287-Ethanol,
pubmed-meshheading:16596287-Gene Expression,
pubmed-meshheading:16596287-I-kappa B Proteins,
pubmed-meshheading:16596287-Interleukin-1,
pubmed-meshheading:16596287-Lipopolysaccharides,
pubmed-meshheading:16596287-Macrophages, Peritoneal,
pubmed-meshheading:16596287-Male,
pubmed-meshheading:16596287-Medicine, Tibetan Traditional,
pubmed-meshheading:16596287-Mice,
pubmed-meshheading:16596287-Mice, Inbred BALB C,
pubmed-meshheading:16596287-Mitogen-Activated Protein Kinases,
pubmed-meshheading:16596287-NF-kappa B,
pubmed-meshheading:16596287-Phosphorylation,
pubmed-meshheading:16596287-Phytotherapy,
pubmed-meshheading:16596287-Plant Extracts,
pubmed-meshheading:16596287-Sepsis,
pubmed-meshheading:16596287-Signal Transduction,
pubmed-meshheading:16596287-Specific Pathogen-Free Organisms,
pubmed-meshheading:16596287-Tumor Necrosis Factor-alpha
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pubmed:year |
2006
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pubmed:articleTitle |
Ethanol extract from Artemisia vestita, a traditional Tibetan medicine, exerts anti-sepsis action through down-regulating the MAPK and NF-kappaB pathways.
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pubmed:affiliation |
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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