Linked Life Data

16543731

Source:http://linkedlifedata.com/resource/pubmed/id/16543731

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
2006-3-17
pubmed:abstractText
It has been reported that epidermal growth factor (EGF) and EGF receptor were highly expressed in embryo, suggesting that the EGF system is related to early embryo development in an autocrine and/or paracrine manner. Glucose becomes the preimplantation exogenous energy substrate and enters the blastocyst via glucose transporters. Thus, the effect of EGF on [3H]-2-deoxyglucose (2-DG) uptake and its related signaling pathways were examined in mouse embryonic stem (ES) cells. EGF significantly increased 2-DG uptake in time- and concentration- dependent manner (>12 hr, >10 ng/ ml) and increased mRNA and protein level of glucose transporter 1 (GLUT1) compared to control, respectively. Actinomycin D and cycloheximide completely blocked the effect of EGF on 2-DG uptake. EGF-induced increase of 2-DG uptake was blocked by AG1478 (EGF receptor tyrosine kinase blocker), genistein or herbimycin (tyrosine kinase inhibitors). In addition, EGF effect was blocked by neomycin and U 73122 [phospholipase C (PLC) inhibitors] as well as staurosporine and bisindolylmaleimide I [protein kinase C (PKC) inhibitors]. EGF was also observed to increase inositol phosphates (IPs) formation and activate a PKC translocation from the cytosolic to membrane fraction, suggesting a role of PLC and PKC. SB 203580 [p38 mitogen activated protein kinase (MAPK) inhibitor] or PD 98059 (p44/42 MAPKs inhibitor) blocked EGF-induced increase of 2-DG uptake. EGF also increased phosphorylation of p38 MAPK and p44/42 MAPKs, which was blocked by genistein or bisindolylmaleimide I, respectively. In conclusion, EGF partially increased 2-DG uptake via PKC, p38 MAPK, and p44/42 MAPKs in mouse ES cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1015-8987
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
145-58
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16543731-Alkaline Phosphatase, pubmed-meshheading:16543731-Animals, pubmed-meshheading:16543731-Antibodies, Monoclonal, pubmed-meshheading:16543731-Antigens, CD15, pubmed-meshheading:16543731-Blotting, Western, pubmed-meshheading:16543731-Cell Line, pubmed-meshheading:16543731-Deoxyglucose, pubmed-meshheading:16543731-Dose-Response Relationship, Drug, pubmed-meshheading:16543731-Epidermal Growth Factor, pubmed-meshheading:16543731-Fluorescein-5-isothiocyanate, pubmed-meshheading:16543731-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:16543731-Fluorescent Dyes, pubmed-meshheading:16543731-Kinetics, pubmed-meshheading:16543731-Luminescence, pubmed-meshheading:16543731-Mice, pubmed-meshheading:16543731-Mitogen-Activated Protein Kinases, pubmed-meshheading:16543731-Models, Biological, pubmed-meshheading:16543731-Protein Kinase C, pubmed-meshheading:16543731-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16543731-Stem Cells, pubmed-meshheading:16543731-Time Factors
pubmed:year
2006
pubmed:articleTitle
PKC and MAPKs pathways mediate EGF-induced stimulation of 2-deoxyglucose uptake in mouse embryonic stem cells.
pubmed:affiliation
Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't