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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1991-9-27
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pubmed:abstractText |
We isolated a human endothelin-1 (ET-1) receptor cDNA from a human placenta cDNA library. The cDNA encodes a 427-amino acid protein with seven putative transmembrane domains. The rank order of the binding to the receptor expressed in COS-7 cells was: ET-1 greater than or equal to ET-2 much greater than ET-3. The receptor expressed in Xenopus oocytes showed a potent electrophysiological response to 1 x 10(-7) M ET-1 under voltage clamp at -60 mV, while a much weaker response was produced by 1 x 10(-7) M ET-3. Northern blot analysis with RNA from human tissues revealed a single band with a size of 4.3 kb in a wide variety of human tissues, especially highly in the blood vessel.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
287
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1652463-Amino Acid Sequence,
pubmed-meshheading:1652463-Animals,
pubmed-meshheading:1652463-Base Sequence,
pubmed-meshheading:1652463-Binding, Competitive,
pubmed-meshheading:1652463-Blotting, Northern,
pubmed-meshheading:1652463-Cell Line,
pubmed-meshheading:1652463-Cloning, Molecular,
pubmed-meshheading:1652463-DNA,
pubmed-meshheading:1652463-Electrophysiology,
pubmed-meshheading:1652463-Endothelins,
pubmed-meshheading:1652463-Gene Expression,
pubmed-meshheading:1652463-Humans,
pubmed-meshheading:1652463-Molecular Sequence Data,
pubmed-meshheading:1652463-Oocytes,
pubmed-meshheading:1652463-Placenta,
pubmed-meshheading:1652463-RNA, Messenger,
pubmed-meshheading:1652463-Receptors, Cell Surface,
pubmed-meshheading:1652463-Receptors, Endothelin,
pubmed-meshheading:1652463-Transfection,
pubmed-meshheading:1652463-Xenopus
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pubmed:year |
1991
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pubmed:articleTitle |
Cloning and expression of human endothelin-1 receptor cDNA.
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pubmed:affiliation |
Department of Medicine, Kyoto University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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