Linked Life Data

16520305

Source:http://linkedlifedata.com/resource/pubmed/id/16520305

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2006-3-7
pubmed:abstractText
We report the generation of Ayu17-449 gene knockout mice in an effort to facilitate studies of its function during mouse development, A special gene trapping vector was transfected into mouse ES cells. The clone with the trapped gene trap was identified by 5' RACE and Southern blot analysis. Ayu17-449 trapped mice were then generated by using this ES clone. In addition, the expression of Ayu17-449 in this mutant line was analyzed with Northern blot. Results showed that in these mutant mice, the trapping vector was located upstream of the Ayu17-449 initiation site, and as a result, the transcription of Ayu17-449 gene was inhibited. Ayu17-449 trapped mice are a useful tool for the analysis of Ayu17-449 gene function in mouse development.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0253-9772
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
129-32
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
[Generation of Ayu17-449 gene knockout mice by gene trapping].
pubmed:affiliation
Key Laboratory of Molecular Infectious Diseases, Chongqing University of Medical Sciences, Chongqing 400016, China. tanghua86162003@yahoo.com.cn
pubmed:publicationType
Journal Article, English Abstract