Source:http://linkedlifedata.com/resource/pubmed/id/16443219
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2006-3-20
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| pubmed:abstractText |
We investigated post-translational modification and subcellular localisation of endogenous platelet-derived growth factor-C (PDGF-C) in human thyroid papillary carcinomas (PTC), non-neoplastic thyroid tissues, and a selection of cultured cell lines. PDGF-C expressed nuclear localisation in 95% of all tested cell types in culture and in 10% of the thyrocytes from both PTC and non-neoplastic tissue. The cell lines expressed two forms of full-length PDGF-C, approximately 39 and approximately 55 kDa, in cell membrane and cytosol, while the approximately 55 kDa form dominated in the nucleus where it was partly chromatin-associated. The approximately 55 kDa form was post-translationally modified by SUMO-1. The putative PDGF-C SUMOylation site is the surface exposed (314)lysine part of a positively charged loop ((312)RPKTGVRGLHK(322)) with characteristics of a nuclear localisation signal. The tissue thyrocytes expressed a non-SUMOylated approximately 43 kDa and the 55 kDa PDGF-C. The SUMO-1 modified approximately 55 kDa PDGF-C expression was low in PTC where the approximately 43 kDa PDGF-C dominated. This is in contrast to non-neoplastic tissue and cultured cells where the SUMOylated approximately 55 kDa PDGF-C was strongly expressed. Our data provide novel evidence for nuclear localisation of PDGF-C, post-translational modification by SUMOylation and the expression of a novel form of PDGF-C in human papillary thyroid carcinomas.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Androstadienes,
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/SUMO-1 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/platelet-derived growth factor C,
http://linkedlifedata.com/resource/pubmed/chemical/wortmannin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
|
| pubmed:issn |
0014-4827
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
312
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
782-95
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16443219-Amino Acid Sequence,
pubmed-meshheading:16443219-Androstadienes,
pubmed-meshheading:16443219-Animals,
pubmed-meshheading:16443219-Carcinoma, Papillary,
pubmed-meshheading:16443219-Cell Line, Tumor,
pubmed-meshheading:16443219-Cell Membrane,
pubmed-meshheading:16443219-Cell Nucleus,
pubmed-meshheading:16443219-Cells, Cultured,
pubmed-meshheading:16443219-Chromatin,
pubmed-meshheading:16443219-Cytosol,
pubmed-meshheading:16443219-Dactinomycin,
pubmed-meshheading:16443219-Gene Expression Regulation,
pubmed-meshheading:16443219-HeLa Cells,
pubmed-meshheading:16443219-Humans,
pubmed-meshheading:16443219-Lymphokines,
pubmed-meshheading:16443219-Mice,
pubmed-meshheading:16443219-Molecular Sequence Data,
pubmed-meshheading:16443219-NIH 3T3 Cells,
pubmed-meshheading:16443219-Platelet-Derived Growth Factor,
pubmed-meshheading:16443219-Protein Conformation,
pubmed-meshheading:16443219-SUMO-1 Protein,
pubmed-meshheading:16443219-Serum,
pubmed-meshheading:16443219-Thyroid Gland,
pubmed-meshheading:16443219-Thyroid Neoplasms
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| pubmed:year |
2006
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| pubmed:articleTitle |
Nuclear localisation of endogenous SUMO-1-modified PDGF-C in human thyroid tissue and cell lines.
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| pubmed:affiliation |
Department of Molecular Biology, University of Bergen, Section of Surgery, Haukeland University Hospital, Bergen 5020, Norway.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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