Linked Life Data

16410064

Source:http://linkedlifedata.com/resource/pubmed/id/16410064

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-1-13
pubmed:abstractText
Tissue type plasminigen activator (t-PA) is one of the important proteolysis factors in the pathogenesis of pulpal inflammation. However, the mechanisms and signal transduction pathways involved in the production of t-PA in human pulp cells are not fully understood. The purpose of this study was to investigate the t-PA activity in human pulp cells stimulated with various pharmacological agents. IL-1alpha was used to evaluate t-PA activity in human pulp cells using casein zymography and enzyme-linked immunosorbent assay (ELISA). Furthermore, to search possible signal transduction pathways, p38 inhibitor SB203580, MEK inhibitor U0126, and phosphatidylinositaol 3-kinase (PI3K) inhibitor LY294002 were added to test how they modulated the t-PA activity. The main casein secreted by human pulp cells migrated at 70 kDa and represented t-PA. Secretion of t-PA was found to be stimulated with IL-1alpha during 2 day cultured period (p < 0.05). From the results of casein zymography and ELISA, SB203580, U0126, and LY294002 significantly reduced the IL-1alpha-stimulated t-PA production, respectively (p < 0.05). Our findings demonstrated that IL-1alpha enhance t-PA production in human pulp cells, and the signal transduction pathways p38, MEK, and PI3K are involved in the inhibition of t-PA. SB203580, U0126, and LY294002 suppress t-PA activity and may also have important implication for pharmacological intervention.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0099-2399
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
30-3
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Examination of the signal transduction pathways leading to upregulation of tissue type plasminogen activator by interleukin-1alpha in human pulp cells.
pubmed:affiliation
Institute of Medicine, College of Oral Medicine, Chung Shan Medical University, Hospital, #110 Section 1 Chien-Kuo N. Road, Taichung, Taiwan.
pubmed:publicationType
Journal Article