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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-1-9
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pubmed:abstractText |
Dendritic cells (DCs) were pulsed with the H-2K(b) binding OVA(257-264)-peptide (SIINFEKL), and used as one single-injection vaccine in combination with anti-CTLA-4 monoclonal antibody (mAb) to treat mice inoculated 3 days previously with 3x10(5) E.G7-OVA lymphoma cells. Neither DC vaccination nor CTLA-4 blockage alone prevented tumor growth in tumor challenged mice. In contrast, the combination of one vaccination and injection of anti-CTLA-4 mAb lead to rejection or retarded tumor growth in more than 60% of the mice. The OVA-transgene or the SIINFEKL-epitope was not lost in the progressing tumors of vaccinated mice, however, the highest degree of anti-SIINFEKL reactivity of host CTLs in an IFN-gamma ELISPOT assay was found only in mice showing complete tumor rejection. Vaccinated mice having rejected E.G7-OVA tumors were capable of rejecting subsequent challenges with 1x10(6) E.G7-OVA tumor cells, and later on these mice even rejected wild-type EL-4 tumor cells indicating that tumor epitope spreading takes place during the process of vaccination-induced E.G7-OVA rejection. In agreement with these observations, mice having rejected E.G7-OVA tumors showed long lasting CTL memory in spleen and bone marrow towards both the SIINFEKL-peptide and other EL-4-derived tumor rejecting epitopes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA-4 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Ctla4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Egg Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/H-2 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/H-2Kb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/OVA-8,
http://linkedlifedata.com/resource/pubmed/chemical/Ovalbumin,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0304-3835
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
231
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
247-56
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16399226-Animals,
pubmed-meshheading:16399226-Antibodies, Monoclonal,
pubmed-meshheading:16399226-Antigens, CD,
pubmed-meshheading:16399226-Antigens, Differentiation,
pubmed-meshheading:16399226-Bone Marrow,
pubmed-meshheading:16399226-CTLA-4 Antigen,
pubmed-meshheading:16399226-Cancer Vaccines,
pubmed-meshheading:16399226-Dendritic Cells,
pubmed-meshheading:16399226-Drug Therapy, Combination,
pubmed-meshheading:16399226-Egg Proteins,
pubmed-meshheading:16399226-Epitopes,
pubmed-meshheading:16399226-Female,
pubmed-meshheading:16399226-H-2 Antigens,
pubmed-meshheading:16399226-Lymphoma,
pubmed-meshheading:16399226-Mice,
pubmed-meshheading:16399226-Mice, Inbred C57BL,
pubmed-meshheading:16399226-Ovalbumin,
pubmed-meshheading:16399226-Peptide Fragments,
pubmed-meshheading:16399226-Signal Transduction,
pubmed-meshheading:16399226-Spleen,
pubmed-meshheading:16399226-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:16399226-Thymoma,
pubmed-meshheading:16399226-Transgenes,
pubmed-meshheading:16399226-Vaccination
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pubmed:year |
2006
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pubmed:articleTitle |
The effect of a therapeutic dendritic cell-based cancer vaccination depends on the blockage of CTLA-4 signaling.
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pubmed:affiliation |
Department of Medical Anatomy, The Panum Institute, The University of Copenhagen, Blegdamsvej 3, Copenhagen 2200N, Denmark. ome@mai.ku.dk
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pubmed:publicationType |
Journal Article
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