Linked Life Data

16359831

Source:http://linkedlifedata.com/resource/pubmed/id/16359831

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-4-3
pubmed:abstractText
A cysteine-rich metal binding protein MT (metallothionein) (named BmtA) is induced upon exposure to multiple heavy metal ions in the freshwater cyanobacterium Oscillatoria brevis. The SmtB/ArsR family repressor BxmR from O. brevis represses the expression of an operon encoding bmtA and bxmR. In the present study, the expression of bmtA was induced in vivo by diamide, a specific thiol oxidant, in O. brevis cells. In vitro electrophoretic gel mobility shift experiments revealed that the incubation with diamide induces disassembly of the BxmR-bxmR/bmtA operator (O)/promoter (P) complex [multiple resolvable complexes of BxmR with oligonucleotide (named P5) containing a single 12-2-12 inverted repeat derived from the O/P region of bxmR/bmtA]. Thus, the exposure to diamide induces MT mRNA in O. brevis, and this induction is associated with diamide-mediated inhibition of BxmR-P5 complex. BxmR is more sensitive to diamide than to H(2)O(2). Furthermore, pretreatment of O. brevis with Zn decreased intracellular peroxidation products caused by diamide. Thus, these results imply that MT induced by Zn-pretreatment functions to protect O. brevis cells against diamide stress.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0378-4274
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
250-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Diamide stress induces a metallothionein BmtA through a repressor BxmR and is modulated by Zn-inducible BmtA in the cyanobacterium Oscillatoria brevis.
pubmed:affiliation
Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't