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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2006-2-7
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pubmed:abstractText |
Viruses require specific cellular receptors to infect their target cells. Angiotensin-converting enzyme 2 (ACE2) is a cellular receptor for two divergent coronaviruses, SARS coronavirus (SARS-CoV) and human coronavirus NL63 (HCoV-NL63). In addition to hostcell receptors, lysosomal cysteine proteases are required for productive infection by some viruses. Here we show that SARS-CoV, but not HCoV-NL63, utilizes the enzymatic activity of the cysteine protease cathepsin L to infect ACE2-expressing cells. Inhibitors of cathepsin L blocked infection by SARS-CoV and by a retrovirus pseudotyped with the SARS-CoV spike (S) protein but not infection by HCoV-NL63 or a retrovirus pseudotyped with the HCoV-NL63 S protein. Expression of exogenous cathepsin L substantially enhanced infection mediated by the SARS-CoV S protein and by filovirus GP proteins but not by the HCoV-NL63 S protein or the vesicular stomatitis virus G protein. Finally, an inhibitor of endosomal acidification had substantially less effect on infection mediated by the HCoV-NL63 S protein than on that mediated by the SARS-CoV S protein. Our data indicate that two coronaviruses that utilize a common receptor nonetheless enter cells through distinct mechanisms.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CTSL1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carboxypeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Cathepsin L,
http://linkedlifedata.com/resource/pubmed/chemical/Cathepsins,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/G protein, vesicular stomatitis...,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptidyl-Dipeptidase A,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/angiotensin converting enzyme 2
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:BoschBerend JanBJ,
pubmed-author:ChoeHyeryunH,
pubmed-author:DermodyTerence STS,
pubmed-author:DormitzerPhilip RPR,
pubmed-author:FarzanMichaelM,
pubmed-author:GhiranSorinaS,
pubmed-author:HarrisonStephen CSC,
pubmed-author:HuangI-ChuehIC,
pubmed-author:LeeKyoung HoaKH,
pubmed-author:LiFangF,
pubmed-author:LiWenhuiW,
pubmed-author:RottierPeter J MPJ,
pubmed-author:VasilievaNatalyaN
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
281
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3198-203
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:16339146-Animals,
pubmed-meshheading:16339146-Carboxypeptidases,
pubmed-meshheading:16339146-Cathepsin L,
pubmed-meshheading:16339146-Cathepsins,
pubmed-meshheading:16339146-Cell Line,
pubmed-meshheading:16339146-Cercopithecus aethiops,
pubmed-meshheading:16339146-Coronavirus,
pubmed-meshheading:16339146-Cysteine Endopeptidases,
pubmed-meshheading:16339146-Endosomes,
pubmed-meshheading:16339146-Green Fluorescent Proteins,
pubmed-meshheading:16339146-Humans,
pubmed-meshheading:16339146-Lysosomes,
pubmed-meshheading:16339146-Membrane Glycoproteins,
pubmed-meshheading:16339146-Peptidyl-Dipeptidase A,
pubmed-meshheading:16339146-Retroviridae,
pubmed-meshheading:16339146-SARS Virus,
pubmed-meshheading:16339146-Species Specificity,
pubmed-meshheading:16339146-Vero Cells,
pubmed-meshheading:16339146-Viral Envelope Proteins
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pubmed:year |
2006
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pubmed:articleTitle |
SARS coronavirus, but not human coronavirus NL63, utilizes cathepsin L to infect ACE2-expressing cells.
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pubmed:affiliation |
Pulmonary Division, Children's Hospital, Children's Hospital Laboratory of Molecular Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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