Source:http://linkedlifedata.com/resource/pubmed/id/16332687
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
2006-2-13
|
| pubmed:abstractText |
Synapse-associated protein 97 (SAP97) and postsynaptic density 95 (PSD-95) are closely related membrane-associated guanylate kinase homologs (Maguks) implicated in the synaptic targeting and anchoring of alpha-amino-5-methyl-3-hydroxy-4-isoxazolepropionic acid (AMPA)-selective glutamate receptors. Prompted by accumulating evidence for an oligomeric nature of Maguks, we examined the potential of SAP97 and PSD-95 to form heteromeric complexes. SAP97 and PSD-95 coimmunoprecipitated from rat brain detergent extracts and subsequent glutathione S-transferase pull-down and immunoprecipitation experiments showed that the interaction is mediated by binding of the N-terminal segment of SAP97 (SAP97(NTD)) to the Src homology 3 domain of PSD-95 (PSD-95(SH3)). In cultured hippocampal neurons, expression of green fluorescent protein-tagged PSD-95 triggered accumulation of SAP97 in synaptic spines, which was totally inhibited by coexpression of PSD-95(SH3). Furthermore, overexpression of green fluorescent protein-PSD-95 induced dendritic clustering of GluR-A subunit-containing AMPA receptors, which was strongly inhibited by cotransfection with SAP97(NTD) and PSD-95(SH3) constructs. Our results demonstrated a direct interaction between SAP97 and PSD-95 and suggested that this association may play a functional role in the trafficking and clustering of AMPA receptors.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/DLG1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Dlgh1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Dlgh1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Dlgh4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Dlgh4 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, AMPA,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glutamate
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
17
|
| pubmed:volume |
281
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4267-73
|
| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:16332687-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:16332687-Animals,
pubmed-meshheading:16332687-Binding Sites,
pubmed-meshheading:16332687-Cell Line,
pubmed-meshheading:16332687-Cells, Cultured,
pubmed-meshheading:16332687-Guanylate Kinase,
pubmed-meshheading:16332687-Humans,
pubmed-meshheading:16332687-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:16332687-Membrane Proteins,
pubmed-meshheading:16332687-Mice,
pubmed-meshheading:16332687-Protein Transport,
pubmed-meshheading:16332687-Rats,
pubmed-meshheading:16332687-Receptors, AMPA,
pubmed-meshheading:16332687-Receptors, Glutamate,
pubmed-meshheading:16332687-Synapses
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Interaction between SAP97 and PSD-95, two Maguk proteins involved in synaptic trafficking of AMPA receptors.
|
| pubmed:affiliation |
Department of Biological and Environmental Sciences, University of Helsinki, Finland.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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