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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2005-12-5
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pubmed:abstractText |
Ginkgetin, a biflavone from Ginkgo biloba leaves, was previously reported to be a phospholipase A(2) inhibitor and this compound showed the potent antiarthritic activity in rat adjuvant-induced arthritis as well as analgesic activity. This investigation was carried out to find effects on cyclooxygenase-2 (COX-2) in vitro effect. Ginkgetin inhibits COX-2 dependent phases of prostaglandin D(2) (PGD(2)) generation in bone marrow-derived mast cells (BMMC) in a concentration-dependent manner with IC(50) values of 0.75 microM. Western blotting probed with specific anti-COX-2 antibodies showed that the decrease in quantity of the PGD(2) product was accompanied by a decrease in the COX-2 protein level. In addition, this compound consistently inhibited the production of leukotriene C(4) (LTC(4)) in a dose dependent manner, with an IC(50) value of 0.33 microM. These results demonstrate that ginkgetin has a dual cyclooxygenase-2/5-lipoxygenase inhibitory activity. Furthermore, this compound also inhibited degranulation reaction in a dose dependent manner, with an IC(50) value of 6.52 microM. Therefore, this compound might provide a basis for novel anti-inflammatory agents.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 5-Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Biflavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2 Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Leukotriene C4,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin D2,
http://linkedlifedata.com/resource/pubmed/chemical/beta-N-Acetylhexosaminidases,
http://linkedlifedata.com/resource/pubmed/chemical/ginkgetin
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0918-6158
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2181-4
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:16327145-Animals,
pubmed-meshheading:16327145-Arachidonate 5-Lipoxygenase,
pubmed-meshheading:16327145-Biflavonoids,
pubmed-meshheading:16327145-Bone Marrow Cells,
pubmed-meshheading:16327145-Cyclooxygenase 2,
pubmed-meshheading:16327145-Cyclooxygenase 2 Inhibitors,
pubmed-meshheading:16327145-Dose-Response Relationship, Drug,
pubmed-meshheading:16327145-Flavonoids,
pubmed-meshheading:16327145-Ginkgo biloba,
pubmed-meshheading:16327145-Interleukin-10,
pubmed-meshheading:16327145-Leukotriene C4,
pubmed-meshheading:16327145-Lipopolysaccharides,
pubmed-meshheading:16327145-Lipoxygenase Inhibitors,
pubmed-meshheading:16327145-Male,
pubmed-meshheading:16327145-Mast Cells,
pubmed-meshheading:16327145-Membrane Proteins,
pubmed-meshheading:16327145-Mice,
pubmed-meshheading:16327145-Mice, Inbred BALB C,
pubmed-meshheading:16327145-Plant Leaves,
pubmed-meshheading:16327145-Prostaglandin D2,
pubmed-meshheading:16327145-Up-Regulation,
pubmed-meshheading:16327145-beta-N-Acetylhexosaminidases
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pubmed:year |
2005
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pubmed:articleTitle |
Ginkgetin, a Biflavone from Ginko biloba leaves, inhibits cyclooxygenases-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells.
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pubmed:affiliation |
College of Pharmacy, Yeungnam University, Gyongsan 712-749, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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