Source:http://linkedlifedata.com/resource/pubmed/id/16280135
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
2006-1-23
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| pubmed:abstractText |
Lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) generation and the concomitant decline in the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) were demonstrated in human monocyte-derived dendritic cells (DC). Further, their relation to the maturation of DC, characterized by the production of cytokines, up-regulation of cell surface molecules and allo-stimulatory capacity, was examined. The LPS-induced ROS generation was demonstrated using electron paramagnetic resonance spectroscopy in intact cells, and was also confirmed using laser scanning confocal microscopy. The GSH/GSSG was assesed using a glutathione assay kit. When the DC were treated with alpha-phenyl-tert-butylnitrone, the ROS generation was attenuated, but the declined GSH/GSSG was not attenuated, and only cytokine production was suppressed among the above-mentioned maturation characteristics. When the DC were treated with glutathione monoethyl ester, both the ROS generation and the declined GSH/GSSG were attenuated, and the maturation characteristics were all suppressed. These findings suggest that the LPS-induced ROS generation and the concomitant decline in GSH/GSSG occur in human monocyte-derived DC and that the former is involved in cytokine production, while the latter is involved in the up-regulation of cell surface molecules and allo-stimulatory capacity. Since the cytokine production and the allo-stimulatory capacity of DC play an important role in inflammatory and immune responses, differential regulation of the ROS generation and the declined GSH/GSSG may be useful as therapeutic tools in diseases where both responses become entangled, such as sepsis and graft-versus-host disease.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic N-Oxides,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrogen Oxides,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/S-ethyl glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/phenyl-N-tert-butylnitrone
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0024-3205
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
25
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| pubmed:volume |
78
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
926-33
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16280135-Antigens, Surface,
pubmed-meshheading:16280135-Cells, Cultured,
pubmed-meshheading:16280135-Cyclic N-Oxides,
pubmed-meshheading:16280135-Cytokines,
pubmed-meshheading:16280135-Dendritic Cells,
pubmed-meshheading:16280135-Flow Cytometry,
pubmed-meshheading:16280135-Glutathione,
pubmed-meshheading:16280135-Humans,
pubmed-meshheading:16280135-Immunoblotting,
pubmed-meshheading:16280135-Indicators and Reagents,
pubmed-meshheading:16280135-Interleukin-12,
pubmed-meshheading:16280135-Lipopolysaccharides,
pubmed-meshheading:16280135-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:16280135-Microscopy, Confocal,
pubmed-meshheading:16280135-Monocytes,
pubmed-meshheading:16280135-Nitric Oxide Synthase Type II,
pubmed-meshheading:16280135-Nitrogen Oxides,
pubmed-meshheading:16280135-Reactive Oxygen Species,
pubmed-meshheading:16280135-Tumor Necrosis Factor-alpha,
pubmed-meshheading:16280135-Up-Regulation
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| pubmed:year |
2006
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| pubmed:articleTitle |
LPS-induced ROS generation and changes in glutathione level and their relation to the maturation of human monocyte-derived dendritic cells.
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| pubmed:affiliation |
Department of Hematology and Oncology, Kyoto University Hospital, Kyoto 606-8507, Japan.
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| pubmed:publicationType |
Journal Article
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