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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0008479,
umls-concept:C0033684,
umls-concept:C0035696,
umls-concept:C0086418,
umls-concept:C0110610,
umls-concept:C0178539,
umls-concept:C0205147,
umls-concept:C0205360,
umls-concept:C0242184,
umls-concept:C0851285,
umls-concept:C1413791
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pubmed:issue |
7
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pubmed:dateCreated |
2006-2-16
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pubmed:abstractText |
Connective tissue growth factor (CTGF/CCN2) can be induced by various forms of stress such as exposure to high glucose, mechanical load, or hypoxia. Here, we investigated the molecular mechanism involved in the induction of ctgf/ccn2 by hypoxia in a human chondrosarcoma cell line, HCS-2/8. Hypoxia increased the ctgf/ccn2 mRNA level by altering the 3'-untranslated region (UTR)-mediated mRNA stability without requiring de novo protein synthesis. After a series of extensive analyses, we eventually found that the cis-repressive element of 84 bases within the 3'-UTR specifically bound to a cytoplasmic/nuclear protein. By conducting a UV crosslinking assay, we found the cytoplasmic/nuclear protein to be a 35 kDa molecule that bound to the cis-element in a hypoxia-inducible manner. These results suggest that a cis-element in the 3'-UTR of ctgf/ccn2 mRNA and trans-factor counterpart(s) play an important role in the post-transcriptional regulation by determining the stability of ctgf/ccn2 mRNA.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3' Untranslated Regions,
http://linkedlifedata.com/resource/pubmed/chemical/CTGF protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Connective Tissue Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Ctgf protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1099-110
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16247469-3' Untranslated Regions,
pubmed-meshheading:16247469-Animals,
pubmed-meshheading:16247469-Bone Neoplasms,
pubmed-meshheading:16247469-Cell Hypoxia,
pubmed-meshheading:16247469-Chondrosarcoma,
pubmed-meshheading:16247469-Connective Tissue Growth Factor,
pubmed-meshheading:16247469-Cytoplasm,
pubmed-meshheading:16247469-Gene Expression Profiling,
pubmed-meshheading:16247469-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:16247469-Humans,
pubmed-meshheading:16247469-Immediate-Early Proteins,
pubmed-meshheading:16247469-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:16247469-Mice,
pubmed-meshheading:16247469-Mice, Nude,
pubmed-meshheading:16247469-Nuclear Proteins,
pubmed-meshheading:16247469-Promoter Regions, Genetic,
pubmed-meshheading:16247469-Protein Biosynthesis,
pubmed-meshheading:16247469-RNA, Messenger,
pubmed-meshheading:16247469-RNA Stability,
pubmed-meshheading:16247469-Tumor Cells, Cultured
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pubmed:year |
2006
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pubmed:articleTitle |
Hypoxic regulation of stability of connective tissue growth factor/CCN2 mRNA by 3'-untranslated region interacting with a cellular protein in human chondrosarcoma cells.
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pubmed:affiliation |
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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