Source:http://linkedlifedata.com/resource/pubmed/id/16233871
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
2005-10-19
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pubmed:abstractText |
Alginates comprised of essentially alternating units of mannuronic (M) acid-guluronic (G) acid (MG-alginate), and G-blocks isolated from a seaweed where subjected to partial acid hydrolysis at pH 3.5 The chain-length distribution of oligosaccharides in the hydrolysate were investigated by statistical analysis after their separation with high-performance anion-exchange chromatography and pulsed amperometric detection (HPAEC-PAD). Simulated depolymerisation of the MG-alginate provided an estimate of the ratio between two acid hydrolysis rate constants (p=8.3+/-1) and the average distribution of the MM linkages in the original sample of polysaccharide chains. In conclusion, we found HPAEC-PAD together with statistical analysis was a useful method to investigate the fine structure and some properties of binary polysaccharides.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9673
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
1093
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
59-68
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pubmed:dateRevised |
2009-1-15
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pubmed:meshHeading |
pubmed-meshheading:16233871-Alginates,
pubmed-meshheading:16233871-Anion Exchange Resins,
pubmed-meshheading:16233871-Carbohydrate Conformation,
pubmed-meshheading:16233871-Carbohydrate Sequence,
pubmed-meshheading:16233871-Chromatography, High Pressure Liquid,
pubmed-meshheading:16233871-Chromatography, Ion Exchange,
pubmed-meshheading:16233871-Electrochemistry,
pubmed-meshheading:16233871-Molecular Sequence Data,
pubmed-meshheading:16233871-Oligosaccharides
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pubmed:year |
2005
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pubmed:articleTitle |
Application of high-performance anion-exchange chromatography with pulsed amperometric detection and statistical analysis to study oligosaccharide distributions--a complementary method to investigate the structure and some properties of alginates.
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pubmed:affiliation |
Norwegian Biopolymer Laboratory (NOBIPOL), Department of Biotechnology, Norwegian University of Science and Technology (NTNU), Sem Saelands vei 6/8, N-7491 Trondheim, Norway. simon.ballance@biotech.ntnu.no
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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