Linked Life Data

16220660

Source:http://linkedlifedata.com/resource/pubmed/id/16220660

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-10-13
pubmed:abstractText
Rat cortical neurons cultured in DMEM/F-12 in the absence of fetal calf serum were harvested on days 3, 6 and 9 in vitro (DIV), followed by homogenization and subsequent SDS-PAGE for immunoblotting using an antibody against MAP-2 or GFAP. Expression of MAP-2 was not markedly changed during cultivation for 3 to 9 DIV, while GFAP was profoundly expressed after 6 DIV in a manner dependent on the duration of culturing. Cortical neurons cultured for 3 DIV were pre-incubated with fluo-3 AM and then subjected to fluorescence image analysis using a confocal microscope. The exposure to NMDA markedly increased the number of neurons with high fluorescence intensity in the absence of MgCl2, without prominently affecting that in the presence of MgCl2. The addition of FeCI2, but not hemoglobin and transferrin, markedly reduced the increase in a concentration-dependent manner in the presence of NMDA. This inhibition by FeCl2 was not affected by the addition of dithiothreitol or 2-mercaptoethanol. These results suggest that free ferrous ions may selectively prevent Ca2+ influx across NMDA receptor channels in a manner different from that done by the redox site on the channels in cultured rat cortical neurons.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1340-2544
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
105-13
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
[A mechanism for modulation of N-methyl-D-aspartate receptor channels by free ferrous ions in cultured rat cortical neurons].
pubmed:affiliation
Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School of Natural Science and Technology, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan.
pubmed:publicationType
Journal Article, English Abstract