Linked Life Data

16183115

Source:http://linkedlifedata.com/resource/pubmed/id/16183115

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6-7
pubmed:dateCreated
2006-5-1
pubmed:abstractText
PGE2 is involved in initiation and progression of labor in many species. Biosynthesis of PGE2 is mediated by cyclooxygenases (COX) and prostaglandin E synthases (PGES). mPGES-1 and COX-2 form an inducible pathway for PGE2 production in many cell systems. In this study we investigated whether mPGES-1 is involved in cytokine induced PGE2 biosynthesis in human trophoblast cells. We have evaluated the cellular and intracellular co-localization of mPGES-1 and COX-2, as well as cPGES and COX-1 in human trophoblast cells by dual immunofluorescent staining. The effect of IL-1beta on mPGES-1 and COX-2 co-localization, such as would occur with infection, and the regulatory effects of pro-inflammatory cytokines IL-1beta and TNF-alpha on transcriptional activity of mPGES-1 and COX-2 in these cells were also studied. We found that in cultured unstimulated trophoblasts, some cells expressed predominantly either mPGES-1 or COX-2, though there were cells co-expressing both enzymes. With IL-1beta treatment, mPGES-1 and COX-2 became more consistently co-localized. mPGES-1 was not transcriptionally co-induced with COX-2 by the cytokine treatment. We conclude that mPGES-1 is not involved in the inducible COX-2 mediated pathway for PGE2 biosynthesis at the transcriptional level, however, the treatment with IL-1beta results in a higher degree of co-ordination of the mPGES-1 and COX-2 protein immunolocalization, eliciting PGE2 synthesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0143-4004
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
576-86
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16183115-Adult, pubmed-meshheading:16183115-Cell Line, Tumor, pubmed-meshheading:16183115-Cells, Cultured, pubmed-meshheading:16183115-Choriocarcinoma, pubmed-meshheading:16183115-Chorion, pubmed-meshheading:16183115-Cyclooxygenase 2, pubmed-meshheading:16183115-Dinoprostone, pubmed-meshheading:16183115-Dose-Response Relationship, Drug, pubmed-meshheading:16183115-Female, pubmed-meshheading:16183115-Gene Expression Regulation, Enzymologic, pubmed-meshheading:16183115-Humans, pubmed-meshheading:16183115-Interleukin-1, pubmed-meshheading:16183115-Intramolecular Oxidoreductases, pubmed-meshheading:16183115-Membrane Proteins, pubmed-meshheading:16183115-Pregnancy, pubmed-meshheading:16183115-RNA, Messenger, pubmed-meshheading:16183115-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16183115-Trophoblasts, pubmed-meshheading:16183115-Up-Regulation
pubmed:articleTitle
IL-1beta treatment does not co-ordinately up-regulate mPGES-1 and COX-2 mRNA expression, but results in higher degree of cellular and intracellular co-localization of their immunoreactive proteins in human placenta trophoblast cells.
pubmed:affiliation
CIHR Group in Development and Fetal Health, Department of Physiology and Obstetrics, Gynecology and Medicine, University of Toronto, Toronto, Ontario, Canada M5S1A8. marina.premyslova@utoronto.ca
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't