Source:http://linkedlifedata.com/resource/pubmed/id/16123381
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2006-2-1
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pubmed:abstractText |
The SDF-1/CXCR4 axis has been implicated in the chemotaxis, homing, mobilization, and expansion of hematopoietic stem and progenitor cells. We studied the effects of a SDF-1 peptide analogue CTCE-0214 on the survival of cord blood CD34+ cells in culture, expansion, and engraftment of expanded cells in the nonobese diabetic/severe combined immunodeficient (NOD/SCID) mouse model. Our results demonstrated that CTCE-0214 synergized with thrombopoietin (TPO), stem cell factor (SCF), or flt-3 ligand (FL) on the survival of stem and progenitor cells in culture. Adding CTCE-0214 at a low concentration (0.01 ng/ml) for 4 days together with TPO, SCF, and FL significantly enhanced ex vivo expansion of CD34+ cells to subsets of primitive (CD34+CD38- cells, colony-forming unit-mixed [CFU-GEMMs]), erythroid (CFU-Es), myeloid (CFU-GMs), and megakaryocytic (CD61+CD41+ cells, CFU-MKs) progenitors, as well as their multilineage engraftment in NOD/SCID mice. Interestingly, the short exposure of expanded cells to CTCE-0214 (100 and 500 ng/ml) for 4 hours did not increase the quantity of progenitor cells but enhanced their engraftment capacity. The proportion of CD34+ cells expressing surface CXCR4 was decreased, but the overall number of this population increased upon expansion. The small peptide analogue of SDF-1 could be developed for ex vivo expansion and improving engraftment of cord blood transplantation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL12,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl12 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CXCR4,
http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombopoietin,
http://linkedlifedata.com/resource/pubmed/chemical/flt3 ligand protein
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1066-5099
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pubmed:author |
pubmed-author:ChuenCarmen Ka YeeCK,
pubmed-author:FokTai FaiTF,
pubmed-author:GuGoldie Jia-ShiGJ,
pubmed-author:LawPingP,
pubmed-author:LeeShuk ManSM,
pubmed-author:LiChi KongCK,
pubmed-author:LiKarenK,
pubmed-author:MerzoukAhmedA,
pubmed-author:NgPak CheungPC,
pubmed-author:SalariHassanH,
pubmed-author:WongDonaldD,
pubmed-author:YuenPatrick Man PanPM
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pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
55-64
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16123381-Animals,
pubmed-meshheading:16123381-Antigens, CD34,
pubmed-meshheading:16123381-Cell Survival,
pubmed-meshheading:16123381-Chemokine CXCL12,
pubmed-meshheading:16123381-Chemokines, CXC,
pubmed-meshheading:16123381-Cytokines,
pubmed-meshheading:16123381-Dose-Response Relationship, Drug,
pubmed-meshheading:16123381-Drug Synergism,
pubmed-meshheading:16123381-Fetal Blood,
pubmed-meshheading:16123381-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:16123381-Hematopoietic Stem Cells,
pubmed-meshheading:16123381-Membrane Proteins,
pubmed-meshheading:16123381-Mice,
pubmed-meshheading:16123381-Mice, SCID,
pubmed-meshheading:16123381-Receptors, CXCR4,
pubmed-meshheading:16123381-Stem Cell Factor,
pubmed-meshheading:16123381-Thrombopoietin
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pubmed:year |
2006
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pubmed:articleTitle |
Small peptide analogue of SDF-1alpha supports survival of cord blood CD34+ cells in synergy with other cytokines and enhances their ex vivo expansion and engraftment into nonobese diabetic/severe combined immunodeficient mice.
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pubmed:affiliation |
Department of Paediatrics, The Chinese University of Hong Kong, 6th Floor, Clinical Sciences Block, Prince of Wales Hospital, Shatin, NT, Hong Kong, People's Republic of China. lipang@cuhk.edu.hk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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