Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2005-8-22
pubmed:abstractText
Transfectants of human CM and NES2Y beta cell lines and primary islets transfected by FADD-DN (dominant-negative form of Fas-associated death domain), a mutant of FADD and/or a superrepressor of nuclear factor kappaB (NF-kappaB) (AdIkappaB(SA)2), were examined for their susceptibility to the TRAIL (TNF-related apoptosis-inducing ligand)-induced death signal pathway, compared with controls, wild-type cells, and vector transfectants in caspase fluorescence, Western blot, electrophoretic mobility shift, apoptosis, and cytotoxicity assays. FADD-DN inhibited caspase-8 activation induced by TRAIL in the transfectants of CM and NES2Y cells. TRAIL-induced apoptosis and cytotoxicity to the FADD-DN transfectants were decreased in comparison to those responses in controls (CM, p < 0.01 and p < 0.01; NES2Y, p < 0.05, and p < 0.02, respectively). When CM, NES2Y, and primary islet cells were transfected by AdIkappaB(SA)2, TRAIL-induced IkappaB degradation and nuclear translocation of NF-kappaB p50/p65 were blocked. TRAIL-induced apoptosis and cytotoxicity to AdIkappaB(SA)2 transfectants of these cells were also reduced (CM, p < 0.02 and p < 0.02; NES2Y, p < 0.01 and p < 0.01, respectively, and islet p < 0.01 for cytotoxicity). Finally, cytotoxicity induced by TRAIL in CM and NES2Y cells transfected with both FADD-DN and AdIkappaB(SA)2 was reduced, compared with that observed in these cells transfected with either FADD-DN alone or AdIkappaB(SA)2 alone, suggesting that FADD and NF-kappaB have synergistic proapoptotic regulatory effects on the susceptibility of beta cell lines and islet cells to TRAIL-induced destruction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing, http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins, http://linkedlifedata.com/resource/pubmed/chemical/CASP8 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Caspase 8, http://linkedlifedata.com/resource/pubmed/chemical/Caspases, http://linkedlifedata.com/resource/pubmed/chemical/FADD protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Fas-Associated Death Domain Protein, http://linkedlifedata.com/resource/pubmed/chemical/I kappa B beta protein, http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B, http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B p50 Subunit, http://linkedlifedata.com/resource/pubmed/chemical/Sulfasalazine, http://linkedlifedata.com/resource/pubmed/chemical/TNF-Related Apoptosis-Inducing..., http://linkedlifedata.com/resource/pubmed/chemical/TNFSF10 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor RelA, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0198-8859
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
799-809
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16112027-Adaptor Proteins, Signal Transducing, pubmed-meshheading:16112027-Apoptosis, pubmed-meshheading:16112027-Apoptosis Regulatory Proteins, pubmed-meshheading:16112027-Caspase 8, pubmed-meshheading:16112027-Caspases, pubmed-meshheading:16112027-Cell Line, pubmed-meshheading:16112027-Cell Line, Tumor, pubmed-meshheading:16112027-Cells, Cultured, pubmed-meshheading:16112027-Electrophoretic Mobility Shift Assay, pubmed-meshheading:16112027-Fas-Associated Death Domain Protein, pubmed-meshheading:16112027-Humans, pubmed-meshheading:16112027-I-kappa B Proteins, pubmed-meshheading:16112027-Insulin-Secreting Cells, pubmed-meshheading:16112027-Membrane Glycoproteins, pubmed-meshheading:16112027-Mutation, pubmed-meshheading:16112027-NF-kappa B, pubmed-meshheading:16112027-NF-kappa B p50 Subunit, pubmed-meshheading:16112027-Protein Binding, pubmed-meshheading:16112027-Protein Transport, pubmed-meshheading:16112027-Signal Transduction, pubmed-meshheading:16112027-Sulfasalazine, pubmed-meshheading:16112027-TNF-Related Apoptosis-Inducing Ligand, pubmed-meshheading:16112027-Transcription Factor RelA, pubmed-meshheading:16112027-Transfection, pubmed-meshheading:16112027-Tumor Necrosis Factor-alpha
pubmed:year
2005
pubmed:articleTitle
Regulation of TNF-related apoptosis-inducing ligand-mediated death-signal pathway in human beta cells by Fas-associated death domain and nuclear factor kappaB.
pubmed:affiliation
Department of Pediatrics, Faculty of Medicine, University of British Columbia, BC, Vancouver, Canada. daweiou@interchange.ubc.ca
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't