Source:http://linkedlifedata.com/resource/pubmed/id/16093724
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| Predicate | Object |
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| rdf:type | |
| lifeskim:mentions |
umls-concept:C0009017,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0162789,
umls-concept:C0185117,
umls-concept:C0680022,
umls-concept:C0887810,
umls-concept:C0936012,
umls-concept:C1135183,
umls-concept:C1336646,
umls-concept:C1456820,
umls-concept:C1516050,
umls-concept:C1883220,
umls-concept:C2911684
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| pubmed:issue |
1
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| pubmed:dateCreated |
2005-8-11
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| pubmed:databankReference | |
| pubmed:abstractText |
We have cloned the complete coding region of the porcine TNFSF10 gene. The porcine TNFSF10 cDNA has an ORF of 870 nucleotides and shares 85% identity with human TNFSF10, and 75% and 72% identity with rat and mouse Tnfsf10 coding sequences, respectively. The deduced porcine TNFSF10 protein consists of 289 amino acids with the calculated molecular mass of 33.5 kDa and a predicted pI of 8.15. The amino acid sequence similarities correspond to 86, 72 and 70% when compared with human, rat and mouse sequences, respectively. Northern blot analysis detected TNFSF10-specific transcripts (approximately 1.7 kb) in various organs of a 10-week-old pig, suggesting ubiquitous expression. Real-time RT-PCR studies of various organs from fetal (days 73 and 98) and postnatal stages (two weeks, eight months) demonstrated developmental and tissue-specific regulation of TNFSF10 mRNA abundance. The chromosomal location of the porcine TNFSF10 gene was determined by FISH of a specific BAC clone to metaphase chromosomes. This TNFSF10 BAC clone has been assigned to SSC13q34-->q36. Additionally, the localization of the TNFSF10 gene was verified by RH mapping on the porcine IMpRH panel.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1424-859X
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
111
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
74-8
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16093724-Amino Acid Sequence,
pubmed-meshheading:16093724-Animals,
pubmed-meshheading:16093724-Base Sequence,
pubmed-meshheading:16093724-Cloning, Molecular,
pubmed-meshheading:16093724-Conserved Sequence,
pubmed-meshheading:16093724-DNA Primers,
pubmed-meshheading:16093724-Humans,
pubmed-meshheading:16093724-In Situ Hybridization, Fluorescence,
pubmed-meshheading:16093724-Mice,
pubmed-meshheading:16093724-Molecular Sequence Data,
pubmed-meshheading:16093724-Multigene Family,
pubmed-meshheading:16093724-Radiation Hybrid Mapping,
pubmed-meshheading:16093724-Rats,
pubmed-meshheading:16093724-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16093724-Sequence Alignment,
pubmed-meshheading:16093724-Sequence Homology, Amino Acid,
pubmed-meshheading:16093724-Swine,
pubmed-meshheading:16093724-Tumor Necrosis Factor-alpha
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| pubmed:year |
2005
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| pubmed:articleTitle |
Molecular cloning, expression analysis and assignment of the porcine tumor necrosis factor superfamily member 10 gene (TNFSF10) to SSC13q34-->q36 by fluorescence in situ hybridization and radiation hybrid mapping.
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| pubmed:affiliation |
Institute for Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig Maximilian University, Munich, Germany.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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