|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
16
|
|
pubmed:dateCreated |
2005-8-4
|
|
pubmed:abstractText |
Cationic liposomes formulated with neutral 1,2-dimyristoyl-sn-glycero-3-phosphocholine and cationic gemini surfactants were used for transfecting different cell lines with a reporter gene. The efficiency in the transfection has been correlated to the high extent of DNA condensation observed by circular dichroism, condensation shown to depend heavily on the gemini spacer structure. Transfection efficiency was better than that obtained with a commercial lipofection kit.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0022-2623
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
11
|
|
pubmed:volume |
48
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
5378-82
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:16078856-Animals,
pubmed-meshheading:16078856-Butylamines,
pubmed-meshheading:16078856-Cations,
pubmed-meshheading:16078856-Cell Line,
pubmed-meshheading:16078856-Cercopithecus aethiops,
pubmed-meshheading:16078856-Circular Dichroism,
pubmed-meshheading:16078856-Cytomegalovirus,
pubmed-meshheading:16078856-DNA,
pubmed-meshheading:16078856-Dimyristoylphosphatidylcholine,
pubmed-meshheading:16078856-Ethylamines,
pubmed-meshheading:16078856-Genes, Reporter,
pubmed-meshheading:16078856-Green Fluorescent Proteins,
pubmed-meshheading:16078856-Humans,
pubmed-meshheading:16078856-Liposomes,
pubmed-meshheading:16078856-Promoter Regions, Genetic,
pubmed-meshheading:16078856-Quaternary Ammonium Compounds,
pubmed-meshheading:16078856-Rats,
pubmed-meshheading:16078856-Structure-Activity Relationship,
pubmed-meshheading:16078856-Surface-Active Agents,
pubmed-meshheading:16078856-Transfection
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
Efficient transfection of DNA by liposomes formulated with cationic gemini amphiphiles.
|
|
pubmed:affiliation |
Istituto di Metodologie Chimiche del CNR and Dipartimento di Chimica, Università degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
