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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0007634,
umls-concept:C0011306,
umls-concept:C0024432,
umls-concept:C0024518,
umls-concept:C0031437,
umls-concept:C0034693,
umls-concept:C0040300,
umls-concept:C0054961,
umls-concept:C0205307,
umls-concept:C0282637,
umls-concept:C1511938,
umls-concept:C1533691,
umls-concept:C1707455
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pubmed:issue |
8
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pubmed:dateCreated |
2005-7-27
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pubmed:abstractText |
Synovial tissues are frequent sites of inflammatory disorders in which dendritic cells (DCs) may play an important role. This study examines potential antigen-presenting cells obtained from synovium-rich tissues (SRTs) by vascular perfusion of rat hind limbs with collagenase and further enzymatic digestion of the disarticulated hind paws in vitro. The three sub-populations of interest were: CD45+MHC IIhi, mainly CD11c+ and CD163-; CD45+MHC IIlo, mainly CD11c- and CD163+ and CD45+MHC II-, mainly CD11c- and CD163+. Expression of CD11c and CD163 correlated with ruffled cell-surface (CD11c+CD163-) and highly vacuolated cytoplasm (CD11c-CD163+), respectively. Culture of the CD45+CD163- sub-population in granulocyte macrophage colony-stimulating factor (GM-CSF) yielded CD45+MHC IIhi CD11c+CD163- cells with veiled morphology, while the large vacuolated cells that expressed CD163 resembled type A synoviocytes in both surface antigen phenotype and morphology. These results demonstrate that SRTs contain indeterminate cells that can differentiate into mature DCs in vitro in response to GM-CSF, plus mature synovial lining macrophages.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CD163 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0953-8178
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1103-15
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16030130-Animals,
pubmed-meshheading:16030130-Antigens, CD,
pubmed-meshheading:16030130-Antigens, CD45,
pubmed-meshheading:16030130-Antigens, Differentiation, Myelomonocytic,
pubmed-meshheading:16030130-Cell Differentiation,
pubmed-meshheading:16030130-Dendritic Cells,
pubmed-meshheading:16030130-Female,
pubmed-meshheading:16030130-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:16030130-Histocompatibility Antigens Class II,
pubmed-meshheading:16030130-Macrophages,
pubmed-meshheading:16030130-Monocytes,
pubmed-meshheading:16030130-Phenotype,
pubmed-meshheading:16030130-Rats,
pubmed-meshheading:16030130-Rats, Inbred Strains,
pubmed-meshheading:16030130-Receptors, Cell Surface,
pubmed-meshheading:16030130-Recombinant Proteins,
pubmed-meshheading:16030130-Synovial Membrane
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pubmed:year |
2005
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pubmed:articleTitle |
MHC II+ CD45+ cells from synovium-rich tissues of normal rats: phenotype, comparison with macrophage and dendritic cell lineages and differentiation into mature dendritic cells in vitro.
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pubmed:affiliation |
Arthritis Research Laboratory, Hanson Institute for Medical Research, Institute of Medical and Veterinary Science, Adelaide, South Australia.
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pubmed:publicationType |
Journal Article,
In Vitro
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