Source:http://linkedlifedata.com/resource/pubmed/id/16028365
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2005-7-20
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| pubmed:abstractText |
Lipid peroxidation plays a major role in vascular dysfunction and age-related cardiovascular diseases. A major product of lipid peroxidation, tert-butyl hydroperoxide (t-BHP), has been reported to modulate vascular reactivity and cellular signaling. To better understand vascular abnormality, we set out to delineate the activation mechanism of nuclear factor kappa B (NF-kappaB) by t-BHP and the regulation of MAPK in endothelial cells. The results showed that t-BHP induces NF-kappaB activation by an inhibitor of kappaB (IkappaB) phosphorylation through IkappaB kinase (IKK) activation. Our data from this t-BHP study also showed increased p38 MAP kinase and ERK activity; however, interestingly, t-BHP showed no influence on JNK. Pretreatment with the p38 MAP kinase inhibitor, SB203580 and the ERK1/2 inhibitor, PD98059, prevented t-BHP-induced increases in p65 translocation, NF-kappaB luciferase activity, and phospho-IKKalpha/beta. Data suggested that t-BHP induces NF-kappaB activation through the IKK pathway, which involves p38 MAPK and ERK activation. This study illustrates a role of t-BHP in NF-kappaB activation and MAPK related-signaling pathways. The t-BHP-induced activation of NF-kappaB and MAPK could be a major player in vascular dysfunctions, as seen in oxidative stressed responses and the vascular inflammatory process.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/tert-Butylhydroperoxide
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
1071-5762
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
39
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
399-409
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:16028365-Animals,
pubmed-meshheading:16028365-Blotting, Western,
pubmed-meshheading:16028365-Cells, Cultured,
pubmed-meshheading:16028365-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:16028365-Endothelium, Vascular,
pubmed-meshheading:16028365-Enzyme Activation,
pubmed-meshheading:16028365-Enzyme Inhibitors,
pubmed-meshheading:16028365-I-kappa B Kinase,
pubmed-meshheading:16028365-Male,
pubmed-meshheading:16028365-Microscopy, Confocal,
pubmed-meshheading:16028365-Mitogen-Activated Protein Kinases,
pubmed-meshheading:16028365-NF-kappa B,
pubmed-meshheading:16028365-Phosphorylation,
pubmed-meshheading:16028365-Protein-Serine-Threonine Kinases,
pubmed-meshheading:16028365-Rats,
pubmed-meshheading:16028365-Reactive Oxygen Species,
pubmed-meshheading:16028365-Signal Transduction,
pubmed-meshheading:16028365-Transfection,
pubmed-meshheading:16028365-tert-Butylhydroperoxide
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| pubmed:year |
2005
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| pubmed:articleTitle |
Activation mechanisms of endothelial NF-kappaB, IKK, and MAP kinase by tert-butyl hydroperoxide.
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| pubmed:affiliation |
Genetic Engineering Research Institute, Aging Tissue Bank, Pusan National University, Busan 609-735, North Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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