Linked Life Data

15982660

Source:http://linkedlifedata.com/resource/pubmed/id/15982660

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2006-1-9
pubmed:abstractText
The cysteine protease cathepsin L is one of the most potent mammalian elastases and collagenases, widely expressed at basal levels in most tested tissues and cell types, and regulated by pro-inflammatory stimuli. The inflammatory arterial diseases abdominal aortic aneurysm (AAA) and atherosclerosis involve extensive vascular remodeling that requires elastolysis and collagenolysis. This study examined the hypothesis that cathepsin L is over-expressed in human AAA and atherosclerotic lesions and its expression in vascular cell types found in these lesions is regulated by pro-inflammatory cytokines. Immunohistochemical and tissue extract immunoblot analysis demonstrated increased expression of cathepsin L in human AAA and atheromata and localized its expression to lesional smooth muscle cells (SMC), endothelial cells (EC), and macrophages. In primary cultured human SMC, EC, and monocyte-derived macrophages, pro-inflammatory cytokines or growth factors induced the expression of cathepsin L and its activity against extracellular collagen and elastin. Patients with coronary artery stenosis (n=65) had higher serum cathepsin L levels than those without lesions detectable by quantitative coronary angiography (n=30) (1.47+/-0.33 ng/ml versus 0.60+/-0.06 ng/ml, p<0.02). A strong correlation between the percent of stenosis of left anterior descending coronary artery and serum cathepsin L levels in patients with stenosis (R=0.542, p<0.0001), also suggests involvement of cathepsin L in these vascular diseases.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9150
pubmed:author
pubmed:issnType
Print
pubmed:volume
184
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
302-11
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15982660-Animals, pubmed-meshheading:15982660-Aortic Aneurysm, Abdominal, pubmed-meshheading:15982660-Atherosclerosis, pubmed-meshheading:15982660-Cathepsin L, pubmed-meshheading:15982660-Cathepsins, pubmed-meshheading:15982660-Cells, Cultured, pubmed-meshheading:15982660-Cysteine Endopeptidases, pubmed-meshheading:15982660-Endothelium, Vascular, pubmed-meshheading:15982660-Enzyme Precursors, pubmed-meshheading:15982660-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:15982660-Gene Expression Regulation, pubmed-meshheading:15982660-Humans, pubmed-meshheading:15982660-Macrophages, Peritoneal, pubmed-meshheading:15982660-Mice, pubmed-meshheading:15982660-Muscle, Smooth, Vascular, pubmed-meshheading:15982660-Polymerase Chain Reaction, pubmed-meshheading:15982660-RNA, Messenger, pubmed-meshheading:15982660-Saphenous Vein
pubmed:year
2006
pubmed:articleTitle
Cathepsin L expression and regulation in human abdominal aortic aneurysm, atherosclerosis, and vascular cells.
pubmed:affiliation
Department of Cell and Molecular Biology, University of Science and Technology of China, Hefei, China.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural