Source:http://linkedlifedata.com/resource/pubmed/id/15979578
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2005-7-4
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pubmed:abstractText |
In order to investigate if beta-endorphins anti-inflammatory effect in cartilage-damaging states is mediated via tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta), we examined its influence on these two cytokines in vitro. Human articular chondrocytes were obtained from patients undergoing total knee arthroplasty and stimulated with beta-endorphin (60-6000 ng/ml). Protein levels of TNF-alpha and IL-1 beta were measured by ELISA in supernatants from articular chondrocyte cultures. beta-Endorphin significantly increased the levels of IL-1 beta for all concentrations used after 15 min incubation, and when stimulated with 600 and 6000 ng/ml after 24 h incubation. The opioid-induced increase in IL-1 beta was blocked by naltrexone in the group tested. TNF-alpha expression was also significantly stimulated by 60 and 600 ng/ml beta-endorphin after 15 min, an effect blocked by naltrexone in the group tested. These findings indicate that the mechanism of beta-endorphins anti-inflammatory influence in cartilage-damaging states is not apparently mediated via these two cytokines modulation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Narcotics,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygen,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Endorphin
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
333
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1295-9
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pubmed:meshHeading |
pubmed-meshheading:15979578-Cartilage, Articular,
pubmed-meshheading:15979578-Cell Hypoxia,
pubmed-meshheading:15979578-Cells, Cultured,
pubmed-meshheading:15979578-Chondrocytes,
pubmed-meshheading:15979578-Dose-Response Relationship, Drug,
pubmed-meshheading:15979578-Gene Expression Regulation,
pubmed-meshheading:15979578-Humans,
pubmed-meshheading:15979578-Interleukin-1,
pubmed-meshheading:15979578-Narcotics,
pubmed-meshheading:15979578-Oxygen,
pubmed-meshheading:15979578-Tumor Necrosis Factor-alpha,
pubmed-meshheading:15979578-beta-Endorphin
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pubmed:year |
2005
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pubmed:articleTitle |
Opiate regulation of IL-1beta and TNF-alpha in cultured human articular chondrocytes.
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pubmed:affiliation |
Department of Orthopaedics, University Hospital of Northern-Norway, 9038 Tromsø, Norway. nenad.andjelkov@fagmed.uit.no
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pubmed:publicationType |
Journal Article
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