Linked Life Data

15978599

Source:http://linkedlifedata.com/resource/pubmed/id/15978599

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2006-6-9
pubmed:abstractText
The micropipette aspiration (MA) experiment remains a quite widely used micromanipulation technique for quantifying the elastic modulus of cells and, less frequently, of other biological samples. However, moduli estimations derived from MA experiments are only valid if the probed sample is non-adherent to the rigid substrate. This study extends this standard formulation by taking into account the influence of the sample adhesion. Using a finite element analysis of the sample aspiration into the micropipette, we derived a new expression of the aspirated length for linear elastic materials. Our results establish that (i) below a critical value, the thickness h of the probed sample must be considered to get an accurate value of its Young's modulus (ii) this critical value depends both on the Poisson's ratio and on the sample adhesivity. Additionally, we propose a novel method which allows the computation of the intrinsic Young's modulus of the adherent probed sample from its measured apparent elasticity modulus. Thanks to the set of computational graphs we derived from our theoretical analysis, we successfully validate this method by experiments performed on polyacrylamide gels. Interestingly, the original procedure we proposed allows a simultaneous quantification of the Young's modulus and of the Poisson's ratio of the adherent gel. Thus, our revisited analysis of MA experiments extends the application domain of this technique, while contributing to decrease the dispersion of elastic modulus values obtained by this method.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0021-9290
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1677-85
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
An extended modeling of the micropipette aspiration experiment for the characterization of the Young's modulus and Poisson's ratio of adherent thin biological samples: numerical and experimental studies.
pubmed:affiliation
Laboratoire TIMC-IMAG, Equipe DynaCell, CNRS UMR 5525, Institut de l'Ingénierie et de l'Information de Santé, Faculté de Médecine de Grenoble, 38706 La Tronche Cedex, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't