Source:http://linkedlifedata.com/resource/pubmed/id/15974189
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2005-6-24
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pubmed:abstractText |
A biosensor for specific determination of hydrogen peroxide was developed by using homogenized artichoke (Cynara scolymus L.) tissue in combination with a dissolved oxygen probe and applied in determination of hydrogen peroxide in milk samples. Artichoke tissue, which has catalase activity, was immobilized with gelatine by means of glutaraldehyde and fixed on a pretreated teflon membrane. The electrode response was maximum when 0.05 M phosphate buffer was used at pH 7.0 and at 30 degrees C. Upon addition of hydrogen peroxide, the electrode gives a linear response in a concentration range of 5.0-50 x 10(-5) M with a response time of 3 min. The method was also applied to the determination of hydrogen peroxide in milk samples.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
1073-1199
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
637-45
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pubmed:dateRevised |
2006-4-17
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pubmed:meshHeading |
pubmed-meshheading:15974189-Animals,
pubmed-meshheading:15974189-Biosensing Techniques,
pubmed-meshheading:15974189-Cattle,
pubmed-meshheading:15974189-Cell-Free System,
pubmed-meshheading:15974189-Cynara scolymus,
pubmed-meshheading:15974189-Gelatin,
pubmed-meshheading:15974189-Hydrogen Peroxide,
pubmed-meshheading:15974189-Membranes, Artificial,
pubmed-meshheading:15974189-Milk,
pubmed-meshheading:15974189-Polytetrafluoroethylene
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pubmed:year |
2004
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pubmed:articleTitle |
A novel amperometric biosensor based on artichoke (Cynara scolymus L.) tissue homogenate immobilized in gelatin for hydrogen peroxide detection.
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pubmed:affiliation |
Ege University Science Faculty, Chemistry Department, Bornova, Izmir, Turkey. niler@mail.ege.edu.tr
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pubmed:publicationType |
Journal Article
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