Linked Life Data

15972632

Source:http://linkedlifedata.com/resource/pubmed/id/15972632

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-6-23
pubmed:abstractText
More than 70% of decidual lymphocytes are NK cells characterized by CD56(bright)CD16(-) phenotype, but the mechanisms by which these NK cells are recruited in the decidua are still almost unrevealed. In this study, we first analyzed the transcription of 18 chemokine receptors in the first-trimester decidual CD56(bright)CD16(-) NK cells. Among these receptors, CXCR4 and CXCR3 were found highly transcribed, and the expression of CXCR4 was verified in most of the decidual CD56(bright)CD16(-) NK cells by flow cytometry. The first-trimester human trophoblasts were found expressing CXCL12/stromal cell-derived factor 1, the specific ligand of CXCR4, by way of in situ hybridization and immunohistochemistry. The primary cultured trophoblast cells were also found to secrete stromal cell-derived factor 1alpha spontaneously, and its concentration was 384.6 +/- 90.7 pg/ml after the trophoblast cells had been cultured for 60 h. All of the ligands for CXCR3 were below the minimal detectable concentration when trophoblast cells were cultured for up to 48 h. Both recombinant human SDF-1alpha and supernatants of the cultured trophoblast cells exhibited chemotactic activity on decidual CD56(bright)CD16(-) NK cells. Our findings suggest that human first-trimester trophoblast cells produce CXCL12, which in turn chemoattracts decidual CD56(bright)CD16(-) NK cells. This activity could contribute to the recruitment mechanism of decidual lymphocytes, especially CD56(bright)CD16(-) NK cells, in decidua, and may be used at a local level to modulate the immune milieu at the materno-fetal interface.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
175
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
61-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15972632-Antigens, CD56, pubmed-meshheading:15972632-Base Sequence, pubmed-meshheading:15972632-Cell Movement, pubmed-meshheading:15972632-Cells, Cultured, pubmed-meshheading:15972632-Chemokine CXCL12, pubmed-meshheading:15972632-Chemokines, CXC, pubmed-meshheading:15972632-Chemotaxis, pubmed-meshheading:15972632-Decidua, pubmed-meshheading:15972632-Female, pubmed-meshheading:15972632-Humans, pubmed-meshheading:15972632-Immunohistochemistry, pubmed-meshheading:15972632-In Situ Hybridization, pubmed-meshheading:15972632-Killer Cells, Natural, pubmed-meshheading:15972632-Pregnancy, pubmed-meshheading:15972632-Pregnancy Trimester, First, pubmed-meshheading:15972632-Protein Biosynthesis, pubmed-meshheading:15972632-RNA, Messenger, pubmed-meshheading:15972632-Receptors, CXCR4, pubmed-meshheading:15972632-Receptors, Chemokine, pubmed-meshheading:15972632-Receptors, IgG, pubmed-meshheading:15972632-Recombinant Proteins, pubmed-meshheading:15972632-Transcription, Genetic, pubmed-meshheading:15972632-Trophoblasts
pubmed:year
2005
pubmed:articleTitle
Human first-trimester trophoblast cells recruit CD56brightCD16- NK cells into decidua by way of expressing and secreting of CXCL12/stromal cell-derived factor 1.
pubmed:affiliation
Laboratory of Reproductive Immunology, Institute of Obstetrics and Gynecology, Fudan University, Shanghai, China.
pubmed:publicationType
Journal Article, In Vitro