Linked Life Data

1596686

Source:http://linkedlifedata.com/resource/pubmed/id/1596686

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-7-7
pubmed:abstractText
1. The effects of exposure of the ferret trachea in vitro to platelet activating factor (PAF) were examined on methacholine-induced smooth muscle contraction, mucus volume and lysozyme outputs, and albumin transport across the tracheal epithelium. 2. Methacholine (0.1-30 microM) produced concentration-dependent increases in tracheal smooth muscle tone and mucus volume, lysozyme and albumin outputs from the trachea. 3. The concentration-response curves for methacholine-induced smooth muscle contraction, mucus volume and lysozyme outputs were all shifted upwards after exposure of the trachea to PAF (1 microM) with a significant increase in maximum response for each variable. The EC50 values for methacholine-induced smooth muscle contraction and mucus volume output were significantly reduced after PAF exposure suggesting an increase in the potency of methacholine. The concentration-response curve for methacholine-induced albumin output was shifted downwards after PAF exposure with a greatly reduced maximum but no change in the EC50 for methacholine. 4. PAF-induced hyperresponsiveness of methacholine-induced smooth muscle contraction, mucus volume and lysozyme outputs was not affected by indomethacin, FPL55712, or mepyramine and cimetidine, but was prevented by catalase and superoxide dismutase (SOD), and by WEB2086. Similarly, PAF-induced inhibition of methacholine-stimulated albumin output was prevented by catalase and SOD, and by WEB2086. 5. We conclude that PAF induces hyperresponsiveness of ferret tracheal smooth muscle and submucosal gland secretion (including lysozyme secretion from serous cells) to methacholine. This hyperresponsiveness is probably produced by receptor-mediated release of oxygen free-radicals. The inhibition of methacholine-induced albumin flux suggests a loss of epithelial function which is also probably mediated by release of free-radicals. The mechanism by which the free-radicals produce the changes in responsiveness to methacholine, and the cellular source of the free-radicals, remain to be established.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-1596685, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-2131784, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-2282464, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-2328394, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-2333980, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-2476558, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-2508982, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-2745324, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-2873440, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-3005546, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-3009098, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-3592402, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-3594072, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-3664082, http://linkedlifedata.com/resource/pubmed/commentcorrection/1596686-6430375
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
105
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
230-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
PAF-induced muscarinic cholinoceptor hyperresponsiveness of ferret tracheal smooth muscle and gland secretion in vitro.
pubmed:affiliation
Department of Physiology, St George's Hospital Medical School, London.
pubmed:publicationType
Journal Article, In Vitro