Linked Life Data

15965952

Source:http://linkedlifedata.com/resource/pubmed/id/15965952

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-11-3
pubmed:abstractText
To evaluate the role of CXC chemokines CXCL8 (IL8), CXCL10 (IP-10), CXCL12 (SDF-1), and CXCL13 (BCA-1) in bone remodeling, we analyzed their effects on osteoblasts (OBs) obtained from subchondral trabecular bone tissue of osteoarthritis (OA) and post-traumatic (PT) patients. The expression of CXC receptors/ligands (CXCR1/CXCL8, CXCR2/CXCL8, CXCR3/CXCL10, CXCR4/CXCL12, and CXCR5/CXCL13) was analyzed in cultured OBs by flow cytometry and immunocytochemistry. Functional assays on CXC chemokine-treated-OBs in the presence or absence of their specific inhibitors were performed to analyze cellular proliferation and the enzymatic response to chemokine activation. The expression of chemokine ligands/receptors was also confirmed in bone tissue samples by immunohistochemical analysis. Collagen type I and alkaline phosphatase mRNA expression were analyzed on CXCL12- and CXCL13-treated OBs by real-time PCR. OBs from both OA and PT patients expressed high levels of CXCR3 and CXCR5 and lower amounts of CXCR1 and CXCR4. CXCL12 and CXCL13, only in OBs from OA patients, induced a significant proliferation that was also confirmed by specific blocking experiments. Moreover, OBs from OA patients released a higher amount of CXCL13 than those of PT patients while no differences were found for CXCL12. In the remodeling area of bone tissue samples, immunohistochemical analysis confirmed that OBs expressed CXCL12/CXCR4 and CXCL13/CXCR5 both in OA and PT samples. CXCL12 and CXCL13 upregulated collagen type I mRNA expression in OBs from OA patients. These data suggest that CXCL12 and CXCL13 may directly modulate cellular proliferation and collagen type I in OA patients, so contributing to the remodeling process that occurs in the evolution of this disease.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase, http://linkedlifedata.com/resource/pubmed/chemical/CXCL10 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CXCL12 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CXCL13 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL10, http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL12, http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL13, http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC, http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type I, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Chemokine, http://linkedlifedata.com/resource/pubmed/chemical/beta-N-Acetylhexosaminidases
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9541
pubmed:author
pubmed:copyrightInfo
Copyright 2005 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
206
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
78-85
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15965952-Alkaline Phosphatase, pubmed-meshheading:15965952-Cell Proliferation, pubmed-meshheading:15965952-Cells, Cultured, pubmed-meshheading:15965952-Chemokine CXCL10, pubmed-meshheading:15965952-Chemokine CXCL12, pubmed-meshheading:15965952-Chemokine CXCL13, pubmed-meshheading:15965952-Chemokines, CXC, pubmed-meshheading:15965952-Collagen Type I, pubmed-meshheading:15965952-Exocytosis, pubmed-meshheading:15965952-Humans, pubmed-meshheading:15965952-Interleukin-8, pubmed-meshheading:15965952-Osteoarthritis, pubmed-meshheading:15965952-Osteoblasts, pubmed-meshheading:15965952-Phenotype, pubmed-meshheading:15965952-Protein Binding, pubmed-meshheading:15965952-Receptors, Chemokine, pubmed-meshheading:15965952-Tibia, pubmed-meshheading:15965952-beta-N-Acetylhexosaminidases
pubmed:year
2006
pubmed:articleTitle
CXCL12 (SDF-1) and CXCL13 (BCA-1) chemokines significantly induce proliferation and collagen type I expression in osteoblasts from osteoarthritis patients.
pubmed:affiliation
Laboratorio di Immunologia e Genetica, Istituti Ortopedici Rizzoli, Bologna, Italy. labimge@alma.unibo.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't