15933022

Source:http://linkedlifedata.com/resource/pubmed/id/15933022

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0010834, umls-concept:C0013682, umls-concept:C0013935, umls-concept:C0025991, umls-concept:C0040669, umls-concept:C0752001, umls-concept:C1880022
pubmed:issue	6
pubmed:dateCreated	2005-6-3
pubmed:abstractText	Wolbachia spp. are intracellular alpha proteobacteria closely related to Rickettsia. The maternally inherited infections occur in a wide range of invertebrates, causing several reproductive abnormalities, including cytoplasmic incompatibility. The artificial transfer of Wolbachia between hosts (transfection) is used both for basic research examining the Wolbachia-host interaction and for applied strategies that use Wolbachia infections to affect harmful insect populations. Commonly employed transfection techniques use embryonic microinjection to transfer Wolbachia-infected embryo cytoplasm or embryo homogenate. Although microinjections of both embryonic cytoplasm and homogenate have been used successfully, their respective transfection efficiencies (rates of establishing stable germ line infections) have not been directly compared. Transfection efficiency may be affected by variation in Wolbachia quantity or quality within the donor embryos and/or the buffer types used in embryo homogenization. Here we have compared Wolbachia bacteria that originate from different embryonic regions for their competencies in establishing stable germ line infections. The following three buffers were compared for their abilities to maintain an appropriate in vitro environment for Wolbachia during homogenization and injection: phosphate-buffered saline, Drosophila Ringer's buffer, and a sucrose-phosphate-glutamate solution (SPG buffer). The results demonstrate that Wolbachia bacteria from both anterior and posterior embryo cytoplasms are competent for establishing infection, although differing survivorships of injected hosts were observed. Buffer comparison shows that embryos homogenized in SPG buffer yielded the highest transfection success. No difference was observed in transfection efficiencies when the posterior cytoplasm transfer and SPG-homogenized embryo techniques were compared. We discuss the results in relation to intra- and interspecific Wolbachia transfection and the future adaptation of the microinjection technique for additional insects.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/NIH-AI-51533
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-10359795, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-11823204, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-11880639, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-12643996, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-12866789, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-14711652, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-15436424, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-2226200, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-4208545, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-7498772, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-7906391, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-820700, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-8511587, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-8817452, http://linkedlifedata.com/resource/pubmed/commentcorrection/15933022-9725842
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/7605801
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Buffers
pubmed:status	MEDLINE
pubmed:month	Jun
pubmed:issn	0099-2240
pubmed:author	pubmed-author:DobsonStephen LSL, pubmed-author:XiZhiyongZ
pubmed:issnType	Print
pubmed:volume	71
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	3199-204
pubmed:dateRevised	2009-11-18
pubmed:meshHeading	pubmed-meshheading:15933022-Animals, pubmed-meshheading:15933022-Buffers, pubmed-meshheading:15933022-Cytoplasm, pubmed-meshheading:15933022-Drosophila, pubmed-meshheading:15933022-Microinjections, pubmed-meshheading:15933022-Wolbachia
pubmed:year	2005
pubmed:articleTitle	Characterization of Wolbachia transfection efficiency by using microinjection of embryonic cytoplasm and embryo homogenate.
pubmed:affiliation	Department of Entomology, University of Kentucky, S-225 Ag. Science Center North, Lexington, Kentucky 40546, USA.
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural