|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
30
|
|
pubmed:dateCreated |
2005-7-25
|
|
pubmed:abstractText |
Biochemical and biophysical methods are used to show that BMP-7 is secreted as a stable complex consisting of the processed growth factor dimer noncovalently associated with its two prodomain propeptide chains and that the BMP-7 complex is structurally similar to the small transforming growth factor beta (TGFbeta) complex. Because the prodomain of TGFbeta interacts with latent TGFbeta-binding proteins, a family of molecules homologous to the fibrillins, the prodomain of BMP-7 was tested for binding to fibrillin-1 or to LTBP-1. The BMP-7 prodomain and BMP-7 complex, but not the separated growth factor dimer, interact with N-terminal regions of fibrillin-1. This interaction may target the BMP-7 complex to fibrillin microfibrils in the extracellular matrix. Immunolocalization of BMP-7 in tissues like the kidney capsule and skin reveals co-localization with fibrillin. However, BMP-7 immunolocalization in other tissues known to be active sites for BMP-7 signaling is not apparent, suggesting that immunolocalization of BMP-7 in certain tissues represents specific extracellular storage sites. These studies suggest that the prodomains of TGFbeta-like growth factors are important for positioning and concentrating growth factors in the extracellular matrix. In addition, they raise the possibility that prodomains of other TGFbeta-like growth factors interact with fibrillins and/or LTBPs and are also targeted to the extracellular matrix.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BMP7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 7,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Glucosides,
http://linkedlifedata.com/resource/pubmed/chemical/Histidine,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/LTBP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Latent TGF-beta Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ltbp1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/fibrillin,
http://linkedlifedata.com/resource/pubmed/chemical/octyl-beta-D-glucoside
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
29
|
|
pubmed:volume |
280
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
27970-80
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:15929982-Amino Acid Sequence,
pubmed-meshheading:15929982-Animals,
pubmed-meshheading:15929982-Binding Sites,
pubmed-meshheading:15929982-Bone Morphogenetic Protein 7,
pubmed-meshheading:15929982-Bone Morphogenetic Proteins,
pubmed-meshheading:15929982-Cell Line,
pubmed-meshheading:15929982-DNA, Complementary,
pubmed-meshheading:15929982-Dimerization,
pubmed-meshheading:15929982-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15929982-Extracellular Matrix,
pubmed-meshheading:15929982-Glucosides,
pubmed-meshheading:15929982-Histidine,
pubmed-meshheading:15929982-Humans,
pubmed-meshheading:15929982-Hydrogen-Ion Concentration,
pubmed-meshheading:15929982-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:15929982-Kidney,
pubmed-meshheading:15929982-Latent TGF-beta Binding Proteins,
pubmed-meshheading:15929982-Light,
pubmed-meshheading:15929982-Mice,
pubmed-meshheading:15929982-Mice, Inbred BALB C,
pubmed-meshheading:15929982-Microfilament Proteins,
pubmed-meshheading:15929982-Microscopy, Electron,
pubmed-meshheading:15929982-Microscopy, Fluorescence,
pubmed-meshheading:15929982-Molecular Sequence Data,
pubmed-meshheading:15929982-Protein Binding,
pubmed-meshheading:15929982-Protein Structure, Secondary,
pubmed-meshheading:15929982-Protein Structure, Tertiary,
pubmed-meshheading:15929982-Recombinant Proteins,
pubmed-meshheading:15929982-Scattering, Radiation,
pubmed-meshheading:15929982-Shadowing (Histology),
pubmed-meshheading:15929982-Signal Transduction,
pubmed-meshheading:15929982-Transforming Growth Factor beta,
pubmed-meshheading:15929982-Ultraviolet Rays
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
The prodomain of BMP-7 targets the BMP-7 complex to the extracellular matrix.
|
|
pubmed:affiliation |
Shriners Hospital for Children, Portland, Oregon 97239, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
