Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 1
pubmed:dateCreated
2005-9-21
pubmed:abstractText
The cysteine-rich domain of the haemorrhagic metalloproteinase atrolysin A was shown to inhibit collagen-stimulated platelet aggregation and to interact with MG-63 osteosarcoma cells via integrin alpha2beta1 to inhibit adhesion to collagen I. In addition, we demonstrate by solid-phase binding assays that atrolysin A binds to collagen I and to vWF (von Willebrand factor) via exosites in the cysteine-rich domain. Interestingly, the binding site of the cysteine-rich domain on collagen I is distinct from the cell adhesion site, since the incubation of collagen-I-coated plates with the cysteine-rich domain did not prevent the adhesion of MG-63 cells to collagen. Finally, we show by surface plasmon resonance (BIAcore) analyses that the cysteine-rich domain can block vWF binding to collagen I as well as the binding of collagen I to vWF. Taken together, these results indicate that this domain may function as a cell-surface-receptor-binding site and/or a substrate recognition exosite and may thus play a role in the pathologies associated with atrolysin A.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-10486149, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-10527632, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-10620350, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-10666318, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-11098050, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-11284707, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-11368322, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-11967265, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-12186858, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-12188898, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-12388743, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-12411949, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-12514095, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-12869506, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-12963038, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-1385408, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-14519395, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-2506015, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-2745407, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-2817904, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-3060135, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-3101740, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-6266278, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-7674931, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-7733877, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-7831660, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-8571303, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-8645248, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-8817809, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-8822592, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-8914925, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-9016825, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-9045854, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-9062552, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-9148922, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-9210644, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-9331419, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-9553097, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-9759634, http://linkedlifedata.com/resource/pubmed/commentcorrection/15929722-9818177
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1470-8728
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
391
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
69-76
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Function of the cysteine-rich domain of the haemorrhagic metalloproteinase atrolysin A: targeting adhesion proteins collagen I and von Willebrand factor.
More...