Linked Life Data

1587947

Source:http://linkedlifedata.com/resource/pubmed/id/1587947

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-6-22
pubmed:abstractText
The effects of 1,2-propanediol, a cryoprotectant used for the freezing of embryos, were tested on the organization of microtubules and microfilaments in mouse oocytes arrested in metaphase II. At low doses (less than or equal to 1.0 M), 1,2-propanediol induced disorganization of the meiotic spindle but at 1.5 M and higher, it stabilized the spindle. Cytoplasmic asters were observed at all doses tested. An extensive network of free microtubules was observed at 1.0 M and 2.0 M 1,2-propanediol, the former having the stronger disruptive effect on the spindle. Higher doses of 1,2-propanediol (greater than or equal to 1.5 M) caused the oocytes to form cytoplasmic blebs. These blebs lacked detectable cortical microfilaments. In contrast, the microfilament-rich area of the cell cortex overlying the meiotic spindle was not modified.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0268-1161
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
374-8
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Effects of 1,2-propanediol on the cytoskeletal organization of the mouse oocyte.
pubmed:affiliation
Institut Jacques Monod, CNRS, Université Paris 7, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't