Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1992-6-12
|
| pubmed:abstractText |
A sensitive and specific radioimmunoassay for human interleukin-8 (IL-8) was developed using isotopically labeled homogenous natural protein. The detection limit (20% inhibition of 125I-IL-8 binding) was 30 pg/100 microliters; 50% displacement occurred at 140 pg/100 microliters. There was no cross-reactivity with the structurally and functionally related neutrophil-activating peptides 2 and 3 up to 500 ng/100 microliters. The intra- and inter-assay coefficients of variation were 4 and 7%, respectively. In vitro experiments showed that human fibroblasts triggered by interleukin-1, double-stranded RNA or virus release immunoreactive and biologically active IL-8 in a dose- and time-dependent manner. Monocytes produce immunoreactive IL-8 in the 100 ng/ml range when exposed to plant mitogen, bacterial endotoxin, virus or IL-1. Although the radioimmunoassay was more sensitive than the chemotaxis assay (detection limit 0.6 ng/ml versus 10 ng/ml) a correlation between concentrations of immunoreactive IL-8 and neutrophil chemotactic activity in the supernatants from stimulated monocytes and fibroblasts was observed. In synovial fluids from patients with inflammatory joint disease, IL-8 was clearly demonstrable, but there was no correlation between IL-8 levels and general parameters of disease activity (erythrocyte sedimentation rate and serum levels of C-reactive protein). Synovial fluids from patients with rheumatoid arthritis, seropositive for rheumatoid factor, contained significantly higher concentrations of IL-8 than synovial fluids from seronegative rheumatoid arthritis patients and patients with non-rheumatoid arthritis joint inflammation. There was a highly significant correlation between IL-8 levels and serum titers of rheumatoid factor. These findings suggest that the molecular mechanisms underlying joint inflammation may be distinct in different types of arthritis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Double-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Rheumatoid Factor
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0023-6837
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
66
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
512-8
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:1583890-Adult,
pubmed-meshheading:1583890-Aged,
pubmed-meshheading:1583890-Arthritis, Rheumatoid,
pubmed-meshheading:1583890-Chemotaxis, Leukocyte,
pubmed-meshheading:1583890-Female,
pubmed-meshheading:1583890-Fibroblasts,
pubmed-meshheading:1583890-Humans,
pubmed-meshheading:1583890-Interleukin-1,
pubmed-meshheading:1583890-Interleukin-8,
pubmed-meshheading:1583890-Male,
pubmed-meshheading:1583890-Middle Aged,
pubmed-meshheading:1583890-Monocytes,
pubmed-meshheading:1583890-RNA, Double-Stranded,
pubmed-meshheading:1583890-RNA, Viral,
pubmed-meshheading:1583890-Radioimmunoassay,
pubmed-meshheading:1583890-Rheumatoid Factor,
pubmed-meshheading:1583890-Sensitivity and Specificity,
pubmed-meshheading:1583890-Synovial Fluid
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Development and application of a radioimmunoassay for interleukin-8: detection of interleukin-8 in synovial fluids from patients with inflammatory joint disease.
|
| pubmed:affiliation |
Division of Pharmacology, University of Antwerp, Belgium.
|
| pubmed:publicationType |
Journal Article
|